Induced and spontaneous chromosomal aberrations in cultured human leukocytes
department or school: Medicine, Faculty of
department or school: Medical Genetics, Department of
data provider: DSpace
degree campus: UBCV
creator: Andrews, John Charles
issued: 2011-06-17T18:35:51Z
issued: 1969
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The frequency of chromosome breaks was increased in replicate cultures from each of ten individuals when lysergic acid diethylamide at a concentration of 1 ug/ml of culture was added 24 hours prior to the harvest of the cells. The differences between the control and treated cultures ranged from +3.00 to +7.93 with a mean of +4.63, indicating no variation in response between individuals. The breaks were randomly distributed among the seven groups of chromosomes of the complement. No significant difference in either the number of cells with aberrations, or the number of breakage events was observed between cells cultured from a patient with Fanconi's anaemia before and 24 hours after treatment with 250 ug. of growth hormone. Both were significantly increased over the control. After treatment with growth hormone, the number of breaks per aberrant cell was decreased, and the distribution of frequencies of specific types of aberrations was changed. Non-homologous exchange figures were the only two break events observed in cultures from the patient. None were observed in control cells. The distribution of breaks among the seven groups of chromosomes was random. The frequency of chromosome aberrations was increased in cultures from a single individual when treated with 1 ug/ml of mitomycin-C for one hour at the beginning of the culture period. In the treated cultures, 181 breaks were observed in 64 of the 100 cells examined, whereas only 5 breaks were observed in three of the 100 cells scored in the control samples. Forty-seven exchange configurations were observed in the treated cultures, 42.56% of these being non-homologous exchanges. No marker or dicentric chromosomes were observed. Breaks were randomly distributed among the seven groups of chromosomes of the complement.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/35556
note: INDUCED AND SPONTANEOUS CHROMOSOMAL ABERRATIONS IN CULTURED HUMAN LEUKOCYTES by JOHN CHARLES ANDREWS B.Sc, University of B r i t i s h Columbia, 1965 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN UKNKTKS in the D i v i s i o n of Medical Genetics We accept t h i s thesis as conforming to the required standard. THE UNIVERSITY OF BRITISH COLUMBIA MAY 1969 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and Study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the Head of my Department or by his representatives. It is understood that copying or publication of this thes.is for financial gain shall not be allowed without my written permission. Department of The University of British Columbia Vancouver 8, Canada - i I ABSTRACT The frequency of chromosome breaks was increased i n r e p l i -cate cultures from each of ten individuals when l y s e r g i c acid diethylamide at a concentration of 1 ug/ml of culture was added 24 hours p r i o r to the harvest of the c e l l s . The differences between the control and treated cultures ranged from +3.00 to +7.93 with a mean of +4.63, indicating no v a r i a t i o n i n response between i n d i v i d u a l s . The breaks were randomly d i s t r i b u t e d among the seven groups of chromosomes of the complement. No s i g n i f i c a n t difference i n either the number of c e l l s with aberrations, or the number of.breakage events was observed between c e l l s cultured from a patient with Fanconi's anaemia before and 24 hours after treatment with 250 ug. of growth hor-mone. Both were s i g n i f i c a n t l y increased over the contr o l . After treatment with growth hormone, the number of breaks per aberrant c e l l was decreased, and the d i s t r i b u t i o n o…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0102224
language: eng
degree discipline: Medical Genetics
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/35556