Characterization of a mutant strain of murine cytomegalovirus which fails to grow in salivary glands
department or school: Medicine, Faculty of
department or school: Pathology and Laboratory Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Boname, Jessica Miriam
issued: 2011-01-28T00:08:25Z
issued: 1991
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: An understanding of the mechanisms of pathogenesis of cytomegaloviruses is essential for future work towards control and prevention of cytomegalovirus disease. Towards this end, a comparison of the Smith, Vancouver and K-181 strains of murine cytomegalovirus (MCMV), which differ in virulence, revealed differences in growth characteristics in vivo and in vitro. The Vancouver strain of MCMV grows to a limited extent in the spleen while it fails to grow in the salivary glands of inoculated mice (salivary gland growth mutant, Sgg⁻). This mutation probably arose during multiple in vitro passage of the parental Smith strain. In vitro, the Vancouver strain replicated more quickly and produced a greater yield of virus per cycle which resulted in a larger plaque size relative to the Smith or K-181 strains. The Vancouver strain has a 9.4 kb deletion, which spans the XbaI I/L junction of the parental Smith strain (0.960 to 0.995 map units), and a 0.9 kb insertion which maps to the EcoRI K fragment (0.37 to 0.47 map units). Recombinant MCMV was generated by co-transfection of cells with whole Vancouver strain DNA and cloned fragments of wild-type DNA. Three independent Sgg⁺ recombinant viruses were selected by passage of the co-transfected virus through mouse salivary glands. The three recombinants had recovered the DNA deletion, while they maintained the insertion associated with the Vancouver strain. Thus, the genotypic location of the defect responsible for the Sgg⁻ phenotype of the Vancouver strain of MCMV maps to 0.960 to 0.995 map units of the Smith virus genome. In addition, the restoration of this region of the genome correlated with the detection in the recombinants of a 42 kDa protein present during E and L times post infection in Smith- and K-181-infected cells, but not in Vancouver strain-infected cells. The K-181 strain was associated with higher salivary gland titres and a low LD₅₀ titre consistent with the increased virulence of this strain compared with the Smit…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/30926
note: CHARACTERIZATION OF A MUTANT STRAIN OF MURINE CYTOMEGALOVIRUS WHICH FAILS TO GROW IN SALIVARY GLANDS by JESSICA MIRIAM BONAME B.Sc, The University of British Columbia, 1983 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE STUDIES (Division of Medical Microbiology, Department of Pathology) We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA August 1991 ° Jessica Miriam Boname, 1991 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. The University of British Columbia Vancouver, Canada Department Date Cd:4t 111 f DE-6 (2/88) A B S T R A C T An understanding of the mechanisms of pathogenesis of cytomegaloviruses is essential for future work towards control and prevention of cytomegalovirus disease. Towards this end, a comparison of the Smith, Vancouver and K-181 strains of murine cytomegalovirus (MCMV), which differ in virulence, revealed differences in growth characteristics in vivo and in vitro. The Vancouver strain of MCMV grows to a limited extent in the spleen while it fails to grow in the salivary glands of inoculated mice (salivary gland growth mutant, Sgg"). This mutation probably arose during multiple in vitro passage of the parental Smith strain. In vitro, the Vancouver strain replicated more quickly and produced a greater yield of virus per cycle which resulted in a larger plaque size relative to the Smith or K-181 strains. The Vancouver strain has a 9.4 kb deletion, which spans the Xbal…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0100605
language: eng
degree discipline: Pathology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/30926