An analysis of intraovarian sperm interaction in the guppy, Poecilia reticulata
department or school: Science, Faculty of
department or school: Zoology, Department of
data provider: DSpace
degree campus: UBCV
creator: Grove, Bryon Dennis
issued: 2010-03-18T19:02:16Z
issued: 1980
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Although guppy females may store sperm in the ovary for several months, they become sexually receptive for a short time every 22-30 days. Females may remate during these receptive periods and consequently, a potential competition between stored and fresh sperm for oocytes may occur. Earlier studies indicated that sperm from the most recent insemination dominates fertilization of new broods. However, these studies provide no clues to how this apparent sperm precedence occurs. There are three possible explanations. First, sperm from new inseminations may outnumber stored sperm and thus, have a statistical advantage. Second, since fertilization in guppies occurs within a few days of insemination, this timing of insemination (relative to egg maturity) may give fresh sperm an advantage. Finally, the location of fresh sperm in the ovary may give them greater access to mature eggs. In this study, females were artificially inseminated with known numbers of spermatophores to see how the proportion of one male's sperm in a mixed insemination determines its contribution to new broods; 2.) the number of spermatophores in inseminations affects the degree of precedence shown by sperm from re-inseminations; and 3.) the timing of re-insemination relative to the female reproductive cycle affects the degree of precedence shown by re-inseminations. An autosomal, recessive allele was used as a genetic marker to distinguish between different males' sperm. In addition, ovaries of females artificially inseminated twice were examined histologically to locate sperm from each insemination. Sperm from one of the inseminations was radioactively labelled. These experiments indicated that sperm precedence does not necessarily result from a statistical advantage to sperm from a re-insemination. Also sperm from re-inseminations occurring up to a week before a new batch of oocytes is mature have strong precedence over stored sperm, although the degree of precedence may be reduced. Autoradiographica…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/22106
note: Ail ANALYSIS OF IHTBAOVARIAS SPEEM INTERACTIONS IN THE GUPPX, PQECILIA RETICULATA by BBYON DENNIS GBOVE B . S c , The U n i v e r s i t y of B r i t i s h Columbia A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIBEHENTS FOB THE DEGBEE OF BASTES OF SCIENCE i n THE FACULTY OF GRADUATE STUDIES (Zoology) Be accept t h i s t h e s i s as conforming to the r e q u i r e d standard THE UNIVERSITY OF BRITISH COLUMBIA Hay 1980 (c) Byron Dennis Grove, 1980 I n p r e s e n t i n g t h i s t h e s i s i n p a r t i a l f u l f i l m e n t o f t h e r e q u i r e m e n t s f o r an a d v a n c e d d e g r e e a t t h e U n i v e r s i t y o f B r i t i s h C o l u m b i a , I a g r e e t h a t t h e L i b r a r y s h a l l make i t f r e e l y a v a i l a b l e f o r r e f e r e n c e a n d s t u d y . I f u r t h e r a g r e e t h a t p e r m i s s i o n f o r e x t e n s i v e c o p y i n g o f t h i s t h e s i s f o r s c h o l a r l y p u r p o s e s may be g r a n t e d by t h e Head o f my D e p a r t m e n t o r by h i s r e p r e s e n t a t i v e s . I t i s u n d e r s t o o d t h a t c o p y i n g o r p u b l i c a t i o n o f t h i s t h e s i s f o r f i n a n c i a l g a i n s h a l l n o t be a l l o w e d w i t h o u t my w r i t t e n p e r m i s s i o n . D e p a r t m e n t o f ^ n i n g y T h e U n i v e r s i t y o f B r i t i s h C o l u m b i a 2075 W e s b r o o k P l a c e V a n c o u v e r , C a n a d a V6T 1W5 D a t e Q^A^2£^ln Abstract Although guppy females may s t o r e sperm i n the ovary f o r s e v e r a l months, they become s e x u a l l y r e c e p t i v e f o r a s h o r t time every 22-30 days., Females may remate during these r e c e p t i v e p e r i o d s and consequently, a p o t e n t i a l c o m p e t i t i o n between s t o r e d and f r e s h sperm f o r oocytes may occur. E a r l i e r s t u d i e s i n d i c a t e d t h a t sperm from the most r e c e n t i n s e m i n a t i o n dominates f e r t i l i z a t i o n of new broo…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0100181
language: eng
degree discipline: Zoology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/22106