A genetic analysis of mutagen-sensitive mutations on the second chromosome of Drosophila melanogaster
department or school: Science, Faculty of
department or school: Zoology, Department of
data provider: DSpace
degree campus: UBCV
creator: Henderson, Daryl Stewart
issued: 2010-07-14T19:46:59Z
issued: 1987
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Mutagen-sensitive (mus) mutations in Drosophila melanogaster render developing flies hypersensitive to the lethal effects of DNA-damaging agents. In general, mus mutations identify DNA repair-related genes. In this study, 5 new second chromosome mus mutations (mus205B¹, mus208B¹, mus209B¹, mus210B¹ and mus211B¹), selected on the basis of sensitivity to methyl methanesulfonate (MMS), were characterized using a variety of genetic tests. One test measured the MMS-sensitivity of double mutant mus strains compared to their component single mutants. Mutant interactions were examined in 8 double mus and in 2 triple mus strains containing combinations of mus201D¹, mus205B¹, mus208B¹, mus210B¹ and mus211B¹ (or mus211B²). These analyses have revealed predominantly synergistic and epistatic responses to MMS. Taken together with the findings of previous genetic and biochemical studies of Drosophila mus strains, these results suggest that 3 major repair pathways may operate in flies to correct damage caused by MMS. Mutagen cross-sensitivity data and the results of the interaction studies suggest that mus mutations might serve as rapid and sensitive bioassays of somatic genotoxicity caused by mutagens and carcinogens. To explore this possibility, a simple mutagen test system was devised employing triple mutant mus strains. One strain (mus208B¹ mus210B¹ mus211B²) was tested for sensitivity to 14 mutagens/carcinogens and 2 non-carcinogens. Eleven of the mutagens/carcinogens were readily detected as genotoxic. Both non-carcinogens were non-genotoxic. These preliminary results demonstrate the feasibility (and some limitations) of the proposed somatic genotoxicity assay and emphasize the need for further test validation using a larger chemical data base. The temperature-sensitive lethal mutation mus209B¹ was subjected to extensive genetic analyses and to temperature shift experiments during development. This locus was found to encode a product(s) that (1) is essential for viability at…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/26418
note: A GENETIC ANALYSIS OF MUTAGEN-SENSITIVE MUTATIONS ON THE SECOND CHROMOSOME OF DROSOPHILA MELANOGASTER By DARYL STEWART HENDERSON B . S c , The U n i v e r s i t y o f B r i t i s h Columbia, 1980 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE i n THE FACULTY OF GRADUATE STUDIES (Department of Zoology) We accept t h i s t h e s i s as conforming t o the r e q u i r e d standard THE UNIVERSITY OF BRITISH COLUMBIA September 1987 © D a r y l Stewart Henderson In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of The University of British Columbia 1956 Main Mall Vancouver, Canada V6T 1 Y 3 Date OCT" \ D E - 6 ( 3 / 8 1 ) ABSTRACT Mutagen-sensitive (mus) mutations i n Drosophila melanogaster render developing f l i e s hypersensitive to the l e t h a l e f f e c t s of DNA-damaging agents. In general, mus mutations i d e n t i f y DNA r e p a i r - r e l a t e d genes. In t h i s study, 5 new second chromosome mus mutations (mus2_05B1, mus208 B 1. mus209 B 1. mus210 B 1 and mus211 B 1), selected on the basis of s e n s i t i v i t y to methyl methanesulfonate (MMS), were characterized using a v a r i e t y of genetic t e s t s . One t e s t measured the MMS-s e n s i t i v i t y of double mutant mus s t r a i n s compared to t h e i r component single mutants. Mutant interactions were examined i n 8 double mus and i n 2 t r i p l e mus s t r a i n s containing combinations of mus201 D 1. mus205 B 1. mus208 B 1, mus210 B 1 and mus211 B 1 (or mus211 B 2). These…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0096960
language: eng
degree discipline: Zoology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/26418