A ²H-NMR study of the comparative effects of lanosterol and cholesterol on model membranes
department or school: Science, Faculty of
department or school: Botany, Department of
department or school: Zoology, Department of
data provider: DSpace
degree campus: UBCV
creator: Wallace, Julia C.
issued: 2010-06-30T05:15:32Z
issued: 1985
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Suspensions of sn-2 deuterated ²H-DPPC-d31 containing various concentrations of either lanosterol or cholesterol were compared using deuterium nuclear magnetic resonance spectroscopy (²H-NMR) and differential scanning calorimetry (DSC). The alpha face of cholesterol is smooth whereas a hydroxyl group protrudes from the alpha face of lanosterol. At the sterol concentrations studied, (0%, 5%, 10%, 15%), the phase behaviour of lanosterol and cholesterol was similar. M, profiles showed that the gel to liquid crystalline phase transition is broadened with the incorporation of both sterols and that mixed phase regions exist at comparable concentrations and temperatures. Both lanosterol and cholesterol increase the average order of the phospholipids above the transition temperature. Since both lanosterol and cholesterol produce this ordering, it is proposed that the significant structural feature producing this result is the rigidity of the cholestane ring. T[sub 2e] increases with the incorporation of both cholesterol and lanosterol. This could indicate the importance of the rigid ring structure which may modify slow motions such as the collective motions of the phospholipid chains.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/26102
note: A 2H-NMR STUDY OF THE COMPARATIVE EFFECTS OF LANOSTEROL AND B.Sc., U n i v e r s i t y o f New B r u n s w i c k , 1983 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE i n THE FACULTY OF GRADUATE STUDIES DEPARTMENT OF BIOLOGY We a c c e p t t h i s t h e s i s a s c o n f o r m i n g t o t h e r e q u i r e d s t a n d a r d THE UNIVERSITY OF BRITISH COLUMBIA CHOLESTEROL ON MODEL MEMBRANES by J U L I A C. WALLACE OCTOBER 1985 © J u l i a C. W a l l a c e , 1985 I n p r e s e n t i n g t h i s t h e s i s i n p a r t i a l f u l f i l m e n t of t h e r e q u i r e m e n t s f o r an a d v a n c e d d e g r e e a t t h e The U n i v e r s i t y o f B r i t i s h C o l u m b i a , I a g r e e t h a t t h e L i b r a r y s h a l l make i t f r e e l y a v a i l a b l e f o r r e f e r e n c e and s t u d y . I f u r t h e r a g r e e t h a t p e r m i s s i o n f o r e x t e n s i v e c o p y i n g of t h i s t h e s i s f o r s c h o l a r l y p u r p o s e s may be g r a n t e d by t h e Head of my Depa r t m e n t o r by h i s o r h e r r e p r e s e n t a t i v e s . I t i s u n d e r s t o o d t h a t c o p y i n g o r p u b l i c a t i o n o f t h i s t h e s i s f o r f i n a n c i a l g a i n s h a l l n o t be a l l o w e d w i t h o u t my w r i t t e n p e r m i s s i o n . DEPARTMENT OF BIOLOGY The U n i v e r s i t y o f B r i t i s h C o l u m b i a 2075 Wesbrook P l a c e V a n c o u v e r , Canada V6T 1W5 D a t e : JANUARY 1986 A b s t r a c t S u s p e n s i o n s of sn-2 d e u t e r a t e d 2H-DPPC-d31 c o n t a i n i n g v a r i o u s c o n c e n t r a t i o n s of e i t h e r l a n o s t e r o l o r c h o l e s t e r o l were compared u s i n g d e u t e r i u m n u c l e a r m a g n e t i c r e s o n a n c e s p e c t r o s c o p y ( 2H-NMR) and d i f f e r e n t i a l s c a n n i n g c a l o r i m e t r y (DSC). The a l p h a f a c e of c h o l e s t e r o l i s smooth whereas a h y d r o x y l g r …
has type: Thesis/Dissertation
is shown at: 10.14288/1.0096824
language: eng
degree discipline: Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/26102