The effect of chromatin structure on P element-induced male recombination in Drosophila melanogaster
department or school: Science, Faculty of
department or school: Zoology, Department of
data provider: DSpace
degree campus: UBCV
creator: Fitzpatrick, Kathleen Anne
issued: 2010-05-13T22:22:00Z
issued: 1985
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Dysgenic male recombination (MR) induced by the P strains T-007 and OKI rarely, if ever, occurs in the heterochromatin of chromosome two. One possible explanation is that the lack of heterochromatic exchange is due to the highly condensed chromatin in this region. Butyrate (a suspected modifier of chromatin structure) induced significant levels of heterochromatic MR in dysgenic hybrids derived from crosses involving two different P strains. This finding is consistent with the hypothesis that chromatin structure can influence the insertion and excision of P elements and hence MR. Analogous experiments were performed using third chromosome suppressor of variegation (Su(var)) mutations. Neither suppressor mutation induced any heterochromatic MR, suggesting that the mode of action of these Su(var) genes is different from, and more specific than, that of butyrate. One of the mutations (325) which is thought to influence meiotic recombination frequencies, causes some alterations in euchromatic MR in crosses involving the OKI strain. The other mutation, 318, affects neither meiotic nor dysgenic recombination. Su(var) 325 is the first known "factor" to influence meiotic and dysgenic recombination similarly.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/24664
note: THE EFFECT OF CHROMATIN STRUCTURE ON P ELEMENT-INDUCED MALE RECOMBINATION IN DROSOPHILA MELANOGASTER BY KATHLEEN ANNE FITZPATRICK B . S c , U n i v e r s i t y of B r i t i s h Columbia, 1980 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE i n THE FACULTY OF GRADUATE STUDIES (Department of Zoology) We accept t h i s t h e s i s as conforming to the r e q u i r e d standard THE UNIVERSITY OF BRITISH COLUMBIA © September, 1985 Kathleen F i t z p a t r i c k , 1985 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of Zoo^O^Y The University of British Columbia 1956 Main Mall Vancouver, Canada V6T 1Y3 DE-6(3/81) i i ABSTRACT Dysgenic male recombination (MR) induced by the P s t r a i n s T-007 and OKI r a r e l y , i f ever, o c c u r s i n the h e t e r o c h r o m a t i n of chromosome two. One p o s s i b l e e x p l a n a t i o n i s that the lack of h e t e r o c h r o m a t i c exchange i s due to the h i g h l y condensed chromatin i n t h i s region. Butyrate (a suspected m o d i f i e r of c h r o m a t i n s t r u c t u r e ) i n d u c e d s i g n i f i c a n t l e v e l s o f h e t e r o c h r o m a t i c MR i n d y s g e n i c h y b r i d s d e r i v e d from c r o s s e s i n v o l v i n g two d i f f e r e n t P s t r a i n s . T h i s f i n d i n g i s c o n s i s t e n with the hypothesis that chromatin s t r u c t u r e can i n f l u e n c e the i n s e r t i o n and e x c i s i o n of P elements and hence MR. Analogous experiments were perf…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0096082
language: eng
degree discipline: Zoology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/24664