Protein synthesis in the ovine fetus
department or school: Land and Food Systems, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Schaefer, Allan Lee
issued: 2010-05-02T23:25:09Z
issued: 1983
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: To study the effect of maternal nutrition on fetal protein synthesis, isotopic kinetic studies were undertaken in which indwelling catheters were implanted in the inferior vena cava and saphenous vein of ovine fetuses as well as the jugular vein and femoral artery of ewes at 120-130 days of gestation Following a five day post surgical recovery period an 8h continuous infusion of L-[2,3,5,6-³H] or L-[U-¹⁴ C] tyrosine was made into the fetus and ewe simultaneously. An enzymatic procedure specific for L-tyrosine was used to measure the plateau specific activity in fetal and maternal plasma. Using these values the net placental transfer, endogenous production and net utilization of tyrosine by the fetus were determined employing a two pool kinetic model. For determining the fractional protein synthetic rates in individual tissues the ewes were sacrificed and the fetuses obtained by laparotomy immediately following the continuous infusion of labelled tyrosine. The effect of starvation on tyrosine transfer across the placenta and protein synthesis by the fetus was studied by repeating the above procedures in ewes starved for 48h. Values for the net placental transfer, endogenous production and net utilization of tyrosine were 5.14, 1.29 and 6.42 mmol/d/kg respectively for fetuses of fed ewes, whereas, these values were -0.29, 4.53 and 3.88 mmol/d/kg in fetuses of starved ewes. The plasma concentrations (mg%) of glucose, alpha amino nitrogen and lactate in the fed ewes were 60.18, 10.68 and 5.58 versus 43.66, 7.80 and 7.41 in starved ewes. Glucose, alpha amino nitrogen and lactate values were also seen to change in the fetus from 12.55, 13.38 and 12.98, respectively in the fed versus 8.18, 11.55 and 15.08 in fetuses of starved ewes. The fetal tyrosine net utilization values, corrected for oxidative loss of 5.2%, were used to calculate the whole body protein synthetic values. From an average tyrosine content of carcass protein of 15.71 mmol/kg, the whole body protein synthe…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/24356
note: PROTEIN SYNTHESIS IN THE OVINE FETUS by ALLAN LEE SCHAEFER B.Sc.(Ag.) r The U n i v e r s i t y of A l b e r t a , 1976 M.Sc.(Ag.), The U n i v e r s i t y of A l b e r t a , 1979 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY i n THE FACULTY OF GRADUATE STUDIES (Department of Animal Science) We accept t h i s t h e s i s as conforming t o the r e q u i r e d standard THE UNIVERSITY OF BRITISH COLUMBIA September 1983 ©Allan Lee Schaefer In presenting t h i s thesis i n p a r t i a l f u l f i l m e n t of the requirements for an advanced degree at the University of B r i t i s h Columbia, I agree that the Library s h a l l make i t f r e e l y available for reference and study. I further agree that permission for extensive copying of t h i s thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. I t i s understood that copying or publication of t h i s thesis for f i n a n c i a l gain s h a l l not be allowed without my written permission. Department of The University of B r i t i s h Columbia 1956 Main Mall Vancouver, Canada V6T 1Y3 Date DE-6 (3/81) i i ABSTRACT To study the e f f e c t of maternal n u t r i t i o n on f e t a l p r o t e i n s y n t h e s i s , i s o t o p i c k i n e t i c s t u d i e s were undertaken i n which i n d w e l l i n g c a t h e t e r s were implanted i n the i n f e r i o r vena cava and saphenous v e i n of ovine f e t u s e s as w e l l as the j u g u l a r v e i n and femoral a r t e r y of ewes at 120-130 days of g e s t a t i o n F o l l o w i n g a f i v e day post s u r g i c a l recovery p e r i o d an 8h 3 14 continuous i n f u s i o n of L-[2,3,5,6"H] or L-[U- C] t y r o s i n e was made i n t o the f e t u s and ewe s i m u l t a n e o u s l y . An enzymatic procedure s p e c i f i c f o r L - t y r o s i n e was used to measure the p l a t e a u s p e c i f i c a c t i v i t y i n f e t a l and maternal plasma. Usin…
has type: Thesis/Dissertation
is shown at: 10.14288/1.0095909
language: eng
degree discipline: Animal Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/24356