Wound healing at the tooth-gingiva interface
department or school: Dentistry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Johnston, Sarah Kate
issued: 2010-01-06T00:34:57Z
issued: 2006
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Healing at the junctional epithelium has been studied at the histological level since early animal and human gingivectomy studies were done in the 1970s (Listgarten et al., 1972, Stahl et al., 1972). Relevance of healing in this area relates to regeneration of the periodontal complex, a goal which has, for the most part, eluded clinical periodontology since that time. On a molecular level there has been little research at the tooth-tissue interface and the present study attempts to shed some light on the similarities and differences in this area compared to other areas in the mouth and rest of the body. Examination of several cell adhesion molecules and their respective ligands, as well as the expression of cytokeratins in the area of the junctional epithelium was investigated between 1 and 60 days post wounding. Cytokeratins 16 and 19 were the earliest molecules expressed. Upregulation of these cytokeratins was evident from the earliest time point, at 1 day post wounding. Expression of CK 19 was also observed to a wider extent in the oral epithelium of periodontitis specimens as well as 1 to 7 day post wounding. Expression of integrins β1, β4, and β6 was most intense between 3 and 7 days post wounding and this paralleled the expression of their respective ligands. In addition, expression of these molecules was usually most intense at a distance from the migratory tip suggesting increased activity coronal to the migrating cells. Finally, the expression of p6 was evident at the junctional epithelium of all specimens examined including healthy tissue samples. Taken together this study provides novel information at a molecular level of the cells of the human junctional epithelium during wounding.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/17588
note: Wound healing at the tooth-gingiva interface by Sarah Kate Johnston DMD, The University of British Columbia, 2000 A Thesis in partial fulfillment of the requirement for the degree Master of Science in The Faculty of Graduate Studies (Dentistry) The University of British Columbia January 2006 © Sarah Kate Johnston, 2006 ABSTRACT: Healing at the junctional epithelium has been studied at the histological level since early animal and human gingivectomy studies were done in the 1970s (Listgarten et al., 1972, Stahl et al., 1972). Relevance of healing in this area relates to regeneration of the periodontal complex, a goal which has, for the most part, eluded clinical periodontology since that time. On a molecular level there has been little research at the tooth-tissue interface and the present study attempts to shed some light on the similarities and differences in this area compared to other areas in the mouth and rest of the body. Examination of several cell adhesion molecules and their respective ligands, as well as the expression of cytokeratins in the area of the junctional epithelium was investigated between 1 and 60 days post wounding. Cytokeratins 16 and 19 were the earliest molecules expressed. Upregulation of these cytokeratins was evident from the earliest time point, at 1 day post wounding. Expression of C K 19 was also observed to a wider extent in the oral epithelium of periodontitis specimens as well as 1 to 7 day post wounding. Expression of integrins p i , P4, and P6 was most intense between 3 and 7 days post wounding and this paralleled the expression of their respective ligands. In addition, expression of these molecules was usually most intense at a distance from the migratory tip suggesting increased activity coronal to the migrating cells. Finally, the expression of p6 was evident at the junctional epithelium of all specimens examined including healthy tissue samples. Taken together this study provides novel information at a molecular level of the c…
has type: Thesis/Dissertation
date issued: 2006-05
is shown at: 10.14288/1.0092500
language: eng
degree discipline: Dental Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/17588