Adamts expression in the human decidua of early pregnancy
department or school: Medicine, Faculty of
department or school: Obstetrics and Gynaecology, Department of
data provider: DSpace
degree campus: UBCV
creator: Ng, York Hunt
issued: 2009-11-24T21:02:51Z
issued: 2004
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The spatio-temporal expression of interleukin (IL)-1β and transforming growth factor (TGF)- β1 in the human endometrium suggests that these two cytokines act in concert with gonadal steroids to create a uterine environment that is capable of supporting a viable pregnancy. The highly regulated series of developmental events that culminates in the terminal differentiation of the stromal cells into deciduas in preparation for implantation is mediated by alterations in the composition of the endometrial extracellular matrix (ECM). Consequently, matrix metalloproteinases have been assigned central roles in implantation and placentation. I hypothesized that members of a novel family of metalloproteinases, the ADAMTS, may mediate certain proteolytic events that are operative at the maternal-fetal interface. A comprehensive survey of the ADAMTS subtypes present in first trimester human decidual tissue was performed. In addition, I have examined the ability of IL-1β and TGF-β1 to regulate the mRNA and protein levels of one of these endometrial ADAMTS subtypes, ADAMTS-1, in primary cultures of stromal cells isolated from first trimester decidua. The mRNA transcripts encoding ADAMTS-1, -2, -3, -4, -5 (-11), -6, -7, -8, -9, -10 and -12 are present in first trimester human deciduas. ADAMTS-1 mRNA transcript and protein expression were detected in all of the decidual stromal cell cultures using quantitative-competitive-polymerase chain reaction (QC-PCR) and Western blotting strategies. IL-βl (0-1000 IU) increased ADAMTS-1 mRNA and protein levels in decidual stromal cell cultures in a dosedependent manner. Significantly increased ADAMTS-1 mRNA and protein levels were also detected in cells cultured for 24 hours in the presence of a fixed amount of IL-1β (100 IU), with maximum levels being observed after 48 hours. In contrast, increasing concentrations of TGF-β1 (0-10 ng) decreased ADAMTS-1 mRNA and protein levels in these cells in a dose-dependent manner. Furthermore, a fixed conc…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/15632
extent: 6487999 bytes
format: application/pdf
note: A D A M T S EXPRESSION IN THE H U M A N DECIDUA OF E A R L Y PREGNANCY B Y Y O R K HUNT N G B.S., O K L A H O M A CITY UNIVERSITY, USA, 2001 A THESIS SUBMITTED TN PARTIAL F U L F I L M E N T OF THE REQUIRMENTS FOR THE DEGREE OF M A S T E R OF SCIENCE IN THE F A C U L T Y OF G R A D U A T E STUDIES THE F A C U L T Y OF MEDICINE DEPARTMENT OF OBSTETRICS A N D G Y N A E C O L O G Y P R O G R A M OF REPRODUCTIVE A N D D E V E L O P M E N T A L SCIENCES WE ACCEPT THIS THESIS AS CONFIRMING TO THE REQUIRED STANDARD THE UNIVERSITY OF BRITISH C O L U M B I A AUGUST 2004 ® Y O R K HUNT N G , 2004 A D A M T S EXPRESSION IN THE H U M A N DECIDUA OF E A R L Y PREGNANCY BY YORK HUNT NG B.S., OKLAHOMA CITY UNIVERSITY, USA, 2001 A THESIS SUBMITTED TN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN THE FACULTY OF GRADUATE STUDIES THE FACULTY OF MEDICINE DEPARTMENT OF OBSTETRICS AND GYNAECOLOGY PROGRAM OF REPRODUCTIVE AND DEVELOPMENTAL SCIENCES WE ACCEPT THIS THESIS AS CONFORMING TO THE REQUIRED STANDARD THE UNIVERSITY OF BRITISH COLUMBIA JULY 2004 ® YORK HUNT NG, 2004 JUBCl THE UNIVERSITY OF BRITISH COLUMBIA FACULTY OF G R A D U A T E STUDIES Library Authorization In presenting this thesis in partial fulfillment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. ZLOOL) Name of Author (please print) Date (dd/mm/yyyy) Title of Thesis: ftP*yrvST£ -p_)( pKgSS\OtO IKS T U E HVJAAftN) P&UIPUft QP BWlM PRfcglNftJKbV' Degree: < M ^ * f CcVuc^ ^ d ^ a w i ^ ^ ^ ^ ^ Q Q ^ •itish Columbia 1-J Department of The University of British Vancouver, BC Canada grad.u…
has type: Thesis/Dissertation
date issued: 2004-05
is shown at: 10.14288/1.0091530
language: eng
degree discipline: Reproductive and Developmental Sciences
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/15632