DNA fingerprinting in Rhododendrons using random amplified polymorphic DNA
department or school: Medicine, Faculty of
department or school: Medical Genetics, Department of
data provider: DSpace
degree campus: UBCV
creator: Sakakibara, Stacey M.
issued: 2009-03-11T19:46:07Z
issued: 1997
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The genus Rhododendron is one of the largest among the angiosperms, and has been the subject of intense interest in the horticultural field for the many species which are of commercial value. Hybrid rhododendrons are also valued, and many thousands of named cultivars have been registered with the International Rhododendron Registry. Rhododendrons make up an important sector of British Columbia's nursery industry, as they thrive in the mild climate of the Lower Mainland and Vancouver Island. The RAPD marker technique was applied in rhododendrons, first for DNA fingerprinting of commercial cultivars, and secondly, to study relationships within the genus For DNA fingerprinting, samples of rhododendron hybrids were obtained from Clay's Nursery in Langley, B.C. Reproducibility of the RAPD technique was established, as was clonal stability of the banding patterns generated. Thirteen RAPD primers were selected and used to generate RAPD banding patterns for the hybrid cultivars. Keys to identification of these cultivars were created based on the presence or absence of certain bands. The reproducibility of RAPDs coupled with the high level of polymorphism generated and the simplicity of the protocol makes the technique a good candidate for use in industry as a tool for quality control and identification of individuals whose identity may be in question. RAPDs were also evaluated for use in parentage analysis in rhododendrons. However, the relatively high frequency of non-parental bands observed limits the usefulness of the technique to certain situations in which there are only a few putative parents. For the taxonomic study, samples were collected from species in subsection Fortunea, as well as from some species thought to be related, and from an outgroup species. Cluster analysis on RAPD data generated from these samples did not give any evidence for the postulated relationships, but did highlight some other relationships. Individuals of the same species were generally clus…
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note: D N A F I N G E R P R I N T I N G IN R H O D O D E N D R O N S U S I N G R A N D O M A M P L I F I E D P O L Y M O R P H I C D N A by S T A C E Y M . S A K A K I B A R A B. Sc., University of British Columbia, Vancouver, British Columbia, 1994 A THESIS S U B M I T T E D IN P A R T I A L F U L F I L L M E N T O F T H E R E Q U I R E M E N T S F O R T H E D E G R E E O F M A S T E R OF S C I E N C E in T H E F A C U L T Y OF G R A D U A T E STUDIES (Genetics Graduate Program) We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA March 1997 Stacey M . Sakakibara, 1997 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the head of my department or by his or her representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of JLyw^^^ . & The University of British Columbia Vancouver, Canada DE-6 (2/88) A B S T R A C T The genus Rhododendron is one of the largest among the angiosperms, and has been the subject of intense interest in the horticultural field for the many species which are of commercial value. Hybrid rhododendrons are also valued, and many thousands of named cultivars have been registered with the International Rhododendron Registry. Rhododendrons make up an important sector of British Columbia's nursery industry, as they thrive in the mild climate of the Lower Mainland and Vancouver Island. The RAPD marker technique was applied in rhododendrons, first for D N A fingerprinting of commercial cultivars, and secondly, to study relationships within the genus For D N A fingerprinting, samples of rhododendron hybrids were obtained from Clay's Nursery in Langley, B…
has type: Thesis/Dissertation
date issued: 1997-05
is shown at: 10.14288/1.0087719
language: eng
degree discipline: Genetics
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/5892