The effect of micromachined surfaces on osteogenesis in vitro
department or school: Dentistry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Ratkay, Julia
issued: 2009-01-17T20:22:16Z
issued: 1995
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Since the use of osseointegrated implants became common in both dentistry and orthopaedics, researchers have been investigating the problem of improving stabilization of the implant. It is widely accepted that the solution lies in improving the biomaterial interface. Implants with various surface designs and chemical compositions have been investigated at the experimental and clinical levels. Micromachining, a technique widely used i n microelectronics, has the capability to produce surface topographies with precise dimensions and high reproducibility. This allows the testing of the effect of implant surface topography on osseointegration in controlled experiments. These experiments aimed to test the ability of micromachined surfaces to promote bone formation in vitro. A recently developed osteogenic culture was optimized and characterized, in which rat calvarial cells were cultured in the presence of ascorbic acid and organic phosphate in the medium. This method provided a reliable model of reproducible mineralized tissue formation. Histochemical methods, TEM, S EM and EDX techniques demonstrated that the mineralized tissue formed after two weeks culturing had an elevated alkalinephosphatase activity, was rich in collagen, and had a structure, similar to the original calvarial bone. The ability of micromachined surfaces to facilitate osteogenesis was studied when these osteogenic cultures were grown on grooved, pitted, and smooth micromachined surfaces. The developed mineralized tissue was stained utilizing von Kossa's technique and its amount quantified with an UltroScan X L Laser Densitometer. The area, shape and orientation of the mineralized bone colonies were measured with a Videoplan Image Analysis System (Zeiss). There was a statistically significant difference of bone formation between the micromachined and smooth surfaces. Bone-like tissue formed to the greatest extent on pitted surfaces, and to a lesser extent on grooves, but few bone nodules were found o…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/3743
extent: 9628210 bytes
format: application/pdf
note: THE E F F E C T OF MICROMACHINED SURFACES O N OSTEOGENESIS IN VITRO by JUL IA RATKAY D.M.D., Semmelweis University Medical School, Budapest, 1986 A THESIS SUBMITTED IN PARTIAL FULF ILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF(.^^9'E:;;IW'. DENTAL SCIENCE in T H E FACULTY OF GRADUATE STUDIES (Department of Oral Biology) We accept this thesis as conforming tathe required standard THE UNIVERSITY OF BRITISH COLUMBIA April, 1995 © Julia Ratkay, 1995 In presenting t h i s thesis i n p a r t i a l f u l f i l l m e n t of the requirements for an advanced degree at the University of B r i t i s h Columbia, I agree that the Library s h a l l make i t f r e e l y a v a i l a b l e for reference and study. I further agree that permission for extensive copying of t h i s thesis for scholarly purposes may be granted by the head of my department or by h i s or her representatives. I t i s understood that copying or publication of t h i s thesis for f i n a n c i a l gain s h a l l not be allowed without my written permission. Department of Oval (LdoUo^ The University of B r i t i s h Columbia Vancouver, Canada Date A p i / c f i 2.6. . Abstract Since the use of osseointegrated implants became common in both dentistry and orthopaedics, researchers have been investigating the problem of improving stabilization of the implant. It is widely accepted that the solution lies in improving the biomaterial interface. Implants with various surface designs and chemical compositions have been investigated at the experimental and clinical levels. Micromachining, a technique widely used in microelectronics, has the capability to produce surface topographies with precise dimensions and high reproducibility. This allows the testing of the effect of implant surface topography on osseointegration in controlled experiments. These experiments aimed to test the ability of micromachined surfaces to promote bone formation in vitro. A recently developed osteogenic culture was optimized and char…
has type: Thesis/Dissertation
date issued: 1995-05
is shown at: 10.14288/1.0086739
language: eng
degree discipline: Dental Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/3743