Rat adrenocortical cell differentiation : effects of signal transduction alterations, ras oncogene expression, and parenchymal/stromal interactions
department or school: Medicine, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Roskelley, Calvin
issued: 2008-12-18T22:49:10Z
issued: 1992
degree grantor: University of British Columbia
description: The zones of the adrenal cortex contain distinct subpopulations of cells which share a common mesodermal origin and steroidogenic template. How zonal specializations are overlaid upon this template is unknown. During adrenocortical cytogenesis zonal phenotypes are expressed transiently as a cell moves inward from a subcapsular position toward the medulla. In this study, homogeneous populations of rat adrenocortical parenchymal cells were maintained and manipulated in vitro. This was done in an effort to determine the mechanism responsible for zone-specific differential responsiveness to the trophic hormone angiotensin II (Ang II). In addition, the effects of ras oncogene expression and parenchymal/stromal interactions on overall steroidogenic differentiation were assessed. Initially, a method was devised to separate rat adrenocortical cells by density into populations which retain zone specific properties in primary culture. Two different parenchymal populations were obtained. They were designated FASC (1 .034g/mI, 18.0µ cell diameter) and GLOM (1.069g1m1, 11.7 µ. cell diameter). In freshly isolated cell suspensions the physical characteristics and differential steroidogenic responses to adrenocorticotropin and angiotensin II suggested that FASC cells originated predominantly from the zona fasciculata and GLOM cells from the zona glomerulosa. In primary culture, the two populations exhibited different morphologies. FASC cells retained lipid and formed cohesive epithelial monolayers that remained stationary for three weeks. GLOM cells were initially epithelial but rapidly lost lipid, spread, assumed fibroblastic shapes and expressed vimentin. Both cell types were positive for the steroidogenic cytochrome P-450 side chain cleavage enzyme (P-45Oscc). Therefore, the morphological changes observed in GLOM cultures were due to modulation, not fibroblastic overgrowth. Ang II increased steroidogenesis in GLOM cells but not in FASC cells. To determine the mechanism responsib…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/3181
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note: RAT ADRENOCORTICAL CELL DIFFERENTIATION: EFFECTS OF SIGNALTRANSDUCTION ALTERATIONS, rasONCOGENE EXPRESSION,AND PARENCHYMAL/STROMAL INTERACTIONSbyCALVIN ROSKELLEYB.Sc., The University of Victoria, 1983A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTSFOR ThE DEGREE OF DOCTOR OF PHILOSOPHYinTHE FACULTY OF GRADUATE STUDIES(Faculty of Medicine, Department of Anatomy)We accept this thesis as conforming to the required standardTHE UNIVERSITY OF BRITISH COLUMBIAMay 1992© Calvin D. RoskelleySignature(s) removed to protect privacyIn presenting this thesis in partial fulfilment of the requirements for an advanceddegree at the University of British Columbia, I agree that the Library shall make itfreely available for reference and study. I further agree that permission for extensivecopying of this thesis for scholarly purposes may be granted by the head of mydepartment or by his or her representatives, It is understood that copying orpublication of this thesis for financial gain shall not be allowed without my writtenpermission.(Signature)_____________________Department of ANATOMYThe University of British ColumbiaVancouver, CanadaDate June 1, 1992DE-6 (2/88)Signature(s) removed to protect privacyIIABSTRACTThe zones of the adrenal cortex contain distinct subpopulations of cells whichshare a common mesodermal origin and steroidogenic template. How zonal specializationsare overlaid upon this template is unknown. During adrenocortical cytogenesis zonalphenotypes are expressed transiently as a cell moves inward from a subcapsularposition toward the medulla. In this study, homogeneous populations of rat adrenocorticalparenchymal cells were maintained and manipulated in vitro. This was done in an effortto determine the mechanism responsible for zone-specific differential responsiveness tothe trophic hormone angiotensin II (Ang II). In addition, the effects of ras oncogeneexpression and parenchymal/stromal interactions on overall steroidogenicdifferentiation were assessed.In…
has type: Thesis/Dissertation
date issued: 1992-11
is shown at: 10.14288/1.0086584
language: eng
degree discipline: Anatomy, Cell Biology and Physiology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/3181