Single-Cell Sequencing: An Emerging Tool for Biomarker Development in Nuclear Emergencies and Radiation Oncology
department or school: Medicine, Faculty of
department or school: Non UBC
data provider: DSpace
identifier citation: Cancers 17 (11): 1801 (2025)
creator: Yu, Jihang
creator: Khan, Md Gulam Musawwir
creator: Mayassi, Nada
creator: Kaushal, Bhuvnesh
creator: Wang, Yi
issued: 2025-06-12T18:55:13Z
issued: 2025-05-28
description: Next-generation sequencing (NGS) has been well applied to assess genetic abnormalities in various biological samples to investigate disease mechanisms. With the advent of high-throughput and automatic testing platforms, NGS can identify radiation-sensitive and dose-responsive biomarkers, contributing to triage patients and determining risk groups for treatment in a nuclear emergency. While bulk NGS provides a snapshot of the average gene expression or genomic changes within a group of cells after the radiation, it cannot provide information on individual cells within the population. On the other hand, single-cell sequencing involves isolating individual cells and sequencing the genetic material from each cell separately. This approach allows for the identification of gene expression and genomic changes in individual cells, providing a high-resolution view of cellular diversity and heterogeneity within a sample. Single-cell sequencing is particularly useful to identify cell-specific features of dose-response and organ-response genes. While single-cell RNA sequencing (scRNA-seq) technology is still emerging in radiation research, it holds significant promise for identifying biomarkers related to radiation exposure and tailoring post-radiation medical care. This review aims to focus on current methods of radiation dosimetry and recently identified biomarkers associated with radiation exposure. Additionally, it addresses the development of NGS techniques in the context of radiation situations, such as cancer treatment and emergency events, with a particular emphasis on single-cell sequencing technology.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/91350
note: Academic Editor: AlexandrosGeorgakilasReceived: 14 March 2025Revised: 24 May 2025Accepted: 25 May 2025Published: 28 May 2025Citation: Yu, J.; Khan, M.G.M.;Mayassi, N.; Kaushal, B.; Wang, Y.Single-Cell Sequencing: An EmergingTool for Biomarker Development inNuclear Emergencies and RadiationOncology. Cancers 2025, 17, 1801.https://doi.org/10.3390/cancers17111801Copyright: © 2025 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license(https://creativecommons.org/licenses/by/4.0/).ReviewSingle-Cell Sequencing: An Emerging Tool for BiomarkerDevelopment in Nuclear Emergencies and Radiation OncologyJihang Yu 1,* , Md Gulam Musawwir Khan 1, Nada Mayassi 1,2, Bhuvnesh Kaushal 1,3 and Yi Wang 1,4,*1 Radiobiology and Health, Isotopes, Radiobiology & Environment Directorate (IRED), Canadian NuclearLaboratories, Chalk River, ON K0J 1J0, Canada2 Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 VictoriaStreet, Toronto, ON M5B 2K3, Canada3 Faculty of Medicine, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada4 Department of Biochemistry Microbiology and Immunology, Faculty of Medicine, University of Ottawa,Ottawa, ON K1H 8M5, Canada* Correspondence: jihang.yu@cnl.ca (J.Y.); yi.wang@cnl.ca (Y.W.); Tel.: +1-613-584-3311 (ext. 42362) (J.Y.);+1-613-584-3311 (ext. 42653) (Y.W.)Simple Summary: Single-cell sequencing is a state-of-the-art technology that allows scien-tists to investigate gene activity and DNA damage in various groups of cells. It has foundbroad applications in cancer research, microbiology, neurology, and immunology. Further-more, single-cell sequencing is invaluable for identifying unknown cell types and specificgenetic changes. This capability makes it a powerful tool for developing biomarkers associ-ated with radiation exposure. These biomarkers can facilitate rapid emergency responses,i…
has type: Article
is shown at: 10.14288/1.0449115
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/cancers17111801
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: next-generation sequencing
subject: single-cell sequencing
subject: radiation dosimetry
subject: radiation biomarkers
subject: cellular heterogeneity
type: Text
identifier URI: http://hdl.handle.net/2429/91350