Integrated Transcriptomic and Metabolomic Analysis Reveals Tissue-Specific Flavonoid Biosynthesis and MYB-Mediated Regulation of UGT71A1 in Panax quinquefolius
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Non UBC
department or school: Biology, Department of (Okanagan)
data provider: DSpace
identifier citation: International Journal of Molecular Sciences 26 (6): 2669 (2025)
creator: Wang, Yumeng
creator: Zhang, Jiaxin
creator: Wang, Ping
creator: Li, Yongkang
creator: Wang, Yihan
creator: Yan, Yan
creator: Chi, Junwen
creator: Chen, Jiankang
creator: Lian, Junmei
creator: Piao, Xiangmin
creator: Lei, Xiujuan
creator: Xiao, Ying
creator: Murray, Jeremy
creator: Deyholos, Micheal K.
creator: Wang, Yingping
creator: Di, Peng
creator: Zhang, Jian (Professor of biology)
issued: 2025-05-07T13:55:49Z
issued: 2025-03-16
description: Panax quinquefolius is a globally valued medicinal plant rich in bioactive flavonoids, yet the molecular mechanisms underlying their biosynthesis remain poorly understood. In this study, we integrated transcriptomic and metabolomic analyses to investigate tissue-specific flavonoid accumulation and regulatory networks in roots, leaves, and flowers. Metabolomic profiling identified 141 flavonoid metabolites, with flavones, flavonols, and C-glycosylflavones predominantly enriched in aerial tissues (leaves and flowers), while specific glycosides like tricin 7-O-acetylglucoside showed root-specific accumulation. Transcriptome sequencing revealed 15,551–18,946 DEGs across tissues, and the reliability of the transcriptomic data was validated by qRT-PCR. KEGG and GO annotation analyses suggested that these DEGs may play a crucial role in the biosynthesis and metabolism of secondary metabolites. From the DEGs, UGTs and MYB TFs were identified and subjected to correlation analysis. Functional validation through in vitro enzymatic assays confirmed that PqUGT71A1 catalyzes apigenin and naringenin glycosylation at the 7-OH position. Additionally, subcellular localization and yeast one-hybrid assays demonstrated that PqMYB7 and PqMYB13 interact with the PqUGT71A1 promoter and activate its expression.. This study unveils the spatial dynamics of flavonoid metabolism in P. quinquefolius and establishes a MYB-UGT regulatory axis, providing critical insights for metabolic engineering and bioactive compound optimization in medicinal plants.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/91019
note: Academic Editor: Soulaiman SakrReceived: 26 February 2025Revised: 10 March 2025Accepted: 14 March 2025Published: 16 March 2025Citation: Wang, Y.; Zhang, J.; Wang,P.; Li, Y.; Wang, Y.; Yan, Y.; Chi, J.; Chen,J.; Lian, J.; Piao, X.; et al. IntegratedTranscriptomic and MetabolomicAnalysis Reveals Tissue-SpecificFlavonoid Biosynthesis andMYB-Mediated Regulation ofUGT71A1 in Panax quinquefolius. Int. J.Mol. Sci. 2025, 26, 2669. https://doi.org/10.3390/ijms26062669Copyright: © 2025 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license(https://creativecommons.org/licenses/by/4.0/).ArticleIntegrated Transcriptomic and Metabolomic Analysis RevealsTissue-Specific Flavonoid Biosynthesis and MYB-MediatedRegulation of UGT71A1 in Panax quinquefoliusYumeng Wang 1,† , Jiaxin Zhang 1,†, Ping Wang 1, Yongkang Li 2, Yihan Wang 1, Yan Yan 1, Junwen Chi 1,Jiankang Chen 1, Junmei Lian 1, Xiangmin Piao 1, Xiujuan Lei 1, Ying Xiao 2, Jeremy Murray 3,Micheal K. Deyholos 4 , Yingping Wang 1, Peng Di 1,* and Jian Zhang 4,5,*1 State Local Joint Engineering Research Center of Ginseng Breeding and Application, College of ChineseMedicinal Materials, Jilin Agricultural University, Changchun 130118, China; ping@mails.jlau.edu.cn (P.W.);20231693@mails.jlau.edu.cn (J.C.); chenjk@mails.jlau.edu.cn (J.C.); lian@mails.jlau.edu.cn (J.L.)2 The MOE Key Laboratory for Standardization of Chinese Medicines and the SHTCM Key Laboratory for NewResources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China3 CAS Center for Excellence in Molecular Plant Sciences, 300 Fenglin Road, Shanghai 200032, China4 Department of Biology, University of British Columbia, Okanagan, Kelowna, BC V1V 1V7, Canada5 Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China* Correspondence: …
has type: Article
is shown at: 10.14288/1.0448770
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/ijms26062669
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: flavonoid biosynthesis
subject: American ginseng
subject: Panax quinquefolius
subject: transcriptomic and metabolomic integration study
type: Text
identifier URI: http://hdl.handle.net/2429/91019