Silver Nanoparticles–Chitosan Nanocomposites as Protective Coatings for Dental Remineralization Treatment: An In Vitro Study
department or school: Dentistry, Faculty of
department or school: Medicine, Faculty of
department or school: Non UBC
department or school: Oral Biological and Medical Sciences (OBMS), Department of
data provider: DSpace
identifier citation: Coatings 15 (1): 40 (2025)
creator: de Rodríguez, Katleen A. Aguirre
creator: de González, Wendy Y. Escobar
creator: Castañeda Monroy, Vianney
creator: Murphy, Sean
creator: Martínez-Castañón, Gabriel-Alejandro
creator: Bach, Horacio
creator: Niño-Martínez, Nereyda
issued: 2025-02-03T19:57:12Z
issued: 2025-01-03
description: Research with nanoparticles for the treatment and prevention of dental caries is of special interest given the high prevalence of the disease worldwide. Several studies support the use of nanoparticles associated with materials given their antimicrobial properties and potential demineralization reduction. This study aimed to evaluate the impact of the application of silver nanoparticles (AgNPs) and chitosan gel in combination with commercial fluoride varnish on the remineralization of dental enamel. Ninety-six tooth blocks were macroscopically evaluated via stereomicroscopy, ICDAS II, and laser fluorescence. Enamel blocks were subjected to artificial demineralization and divided into four exposure groups (24, 48, 120, and 168 h), and five different remineralizing agents were applied, namely, FV (fluoride varnish), FV + CG (fluoride varnish + chitosan gel), FV + AgNPs (fluoride varnish + AgNPs), FV + AgNPs + CG (fluoride varnish + AgNPs + chitosan gel), and AgNPs + CG (AgNPs + chitosan gel). Enamel surface changes were evaluated via laser fluorescence, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Laser fluorescence results obtained from demineralized blocks and subsequently exposed to remineralizing treatment indicate significant differences. After exposure to remineralizing agents, hydroxyapatite and modified apatite phases were identified mainly in the samples treated with FV + AgNPs + CG in the groups exposed for 24, 48, and 120 h. The FV + AgNPs + CG and AgNPs + CG indicate good performance in terms of the Ca/P ratio in in vitro demineralization compared to the group treated with fluorine varnish.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/90306
note: Academic Editors: Egemen Avcu,Yasemin Yıldıran Avcu andMert GuneyReceived: 9 December 2024Revised: 31 December 2024Accepted: 2 January 2025Published: 3 January 2025Citation: de Rodríguez, K.A.A.; deGonzález, W.Y.E.; Castañeda Monroy,V.; Murphy, S.; Martínez-Castañón,G.-A.; Bach, H.; Niño-Martínez, N.Silver Nanoparticles–ChitosanNanocomposites as ProtectiveCoatings for Dental RemineralizationTreatment: An In Vitro Study. Coatings2025, 15, 40. https://doi.org/10.3390/coatings15010040Copyright: © 2025 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license(https://creativecommons.org/licenses/by/4.0/).ArticleSilver Nanoparticles–Chitosan Nanocomposites as ProtectiveCoatings for Dental Remineralization Treatment:An In Vitro StudyKatleen A. Aguirre de Rodríguez 1,2 , Wendy Y. Escobar de González 1,2 , Vianney Castañeda Monroy 2,Sean Murphy 3, Gabriel-Alejandro Martínez-Castañón 4,* , Horacio Bach 5 and Nereyda Niño-Martínez 6,*1 Facultad de Estomatología, Zona Universitaria, Av. Dr. Manuel Nava No. 2, San Luis Potosí CP 78290, Mexico;katleen.aguirre@ues.edu.sv (K.A.A.d.R.); wendy.escobar@ues.edu.sv (W.Y.E.d.G.)2 Laboratorio de Biología Molecular, CENSALUD, Universidad de El Salvador (UES),San Salvador CP 1101, El Salvador; vianney.monroy@ues.edu.sv3 Faculty of Dentistry, Department of Oral Biological and Medical Sciences, University of British Columbia,Vancouver, BC V6H 3Z6, Canada; sean.murphy@dentistry.ubc.ca4 Facultad de Estomatología, Universidad Autónoma de San Luis Potosi, Zona Universitaria, Av. Dr. ManuelNava No. 2, San Luis Potosí CP 78290, Mexico5 Faculty of Medicine, Division of Infectious Diseases, University of British Columbia,Vancouver, BC V6H 3Z6, Canada; horacio.bach@ubc.ca6 Facultad de Ciencias, Universidad Autónoma de San Luis Potosi, Av. Parque Chapultepec No. 1570, Privadasdel Pedregal, San Luis Potosí CP 78295, Mexico* Correspo…
has type: Article
is shown at: 10.14288/1.0447913
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/coatings15010040
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: enamel remineralization
subject: demineralization
subject: chitosan
subject: silver nanoparticles
type: Text
identifier URI: http://hdl.handle.net/2429/90306