Osteoregeneration of Critical-Size Defects Using Hydroxyapatite–Chitosan and Silver–Chitosan Nanocomposites
department or school: Medicine, Faculty of
department or school: Non UBC
department or school: Medicine, Department of
data provider: DSpace
identifier citation: Nanomaterials 13 (2): 321 (2023)
creator: Casillas-Santana, Miguel A.
creator: Slavin, Yael N.
creator: Zhang, Peng
creator: Niño-Martínez, Nereyda
creator: Bach, Horacio
creator: Martínez-Castañón, Gabriel A.
issued: 2025-01-31T17:46:15Z
issued: 2023-01-12
description: Bone is a natural nanocomposite composed of proteins and minerals that can regenerate itself. However, there are conditions in which this process is impaired, such as extensive bone defects and infections of the bone or surrounding tissue. This study evaluates the osteoregenerative capacity of bone grafting materials in animals with induced bone defects. Colloidal chitosan dispersion nanocomposites, nanohydroxyapatite–chitosan (NHAP-Q) and nanosilver–chitosan (AgNP-Q), were synthesized and characterized. Non-critical-size defects in Wistar rats were used to evaluate the material’s biocompatibility, and critical-size defects in the calvarias of guinea pigs were used to evaluate the regenerative capacity of the bones. Moreover, the toxicity of the nanocomposites was evaluated in the heart, liver, spleen, kidneys, and skin. Histological, radiographic, and electron microscopy tests were also performed. The results showed that neither material produced pathological changes. Radiographic examination showed a significant reduction in defects (75.1% for NHAP-Q and 79.3% for AgNP-Q), angiogenesis, and trabecular formation. A toxicological assessment of all the organs did not show changes in the ultrastructure of tissues, and the distribution of silver was different for different organs (spleen > skin > heart > kidney > liver). The results suggest that both materials are highly biocompatible, and AgNP-Q achieved similar bone regeneration to that reported with autologous bone. The main research outcome of the present study was the combination of two types of NPs to enhance antimicrobial and osteoregeneration activities. These colloidal chitosan dispersions show promise as future biomaterials in the medical field for applications in fast-healing fractures, including broken bones in the oral cavity and hip replacement infections.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/90248
note: Citation: Casillas-Santana, M.A.;Slavin, Y.N.; Zhang, P.;Niño-Martínez, N.; Bach, H.;Martínez-Castañón, G.A.Osteoregeneration of Critical-SizeDefects UsingHydroxyapatite–Chitosan andSilver–Chitosan Nanocomposites.Nanomaterials 2023, 13, 321. https://doi.org/10.3390/nano13020321Academic Editor: Miguel GamaReceived: 21 December 2022Revised: 8 January 2023Accepted: 9 January 2023Published: 12 January 2023Copyright: © 2023 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).nanomaterialsArticleOsteoregeneration of Critical-Size Defects UsingHydroxyapatite–Chitosan and Silver–Chitosan NanocompositesMiguel A. Casillas-Santana 1 , Yael N. Slavin 2, Peng Zhang 2, Nereyda Niño-Martínez 3, Horacio Bach 2,*and Gabriel A. Martínez-Castañón 1,*1 Laboratorio de Nanobiomateriales, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí,San Luis Potosí 78290, Mexico2 Division of Infectious Diseases, Faculty of Medicine, University of British Columbia,Vancouver, BC V6G 3Z6, Canada3 Facultad de Ciencias, Universidad Autónoma de San Luis Potosi, San Luis Potosí 78295, Mexico* Correspondence: hbach@mail.ubc.ca (H.B.); mtzcastanon@fciencias.uaslp.mx (G.A.M.-C.)Abstract: Bone is a natural nanocomposite composed of proteins and minerals that can regenerateitself. However, there are conditions in which this process is impaired, such as extensive bone defectsand infections of the bone or surrounding tissue. This study evaluates the osteoregenerative capacityof bone grafting materials in animals with induced bone defects. Colloidal chitosan dispersionnanocomposites, nanohydroxyapatite–chitosan (NHAP-Q) and nanosilver–chitosan (AgNP-Q), weresynthesized and characterized. Non-critical-size defects in Wistar rats were used to evaluate the ma-terial’s biocompatibility, and critical-size defects in the calvarias o…
has type: Article
is shown at: 10.14288/1.0447895
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/nano13020321
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: silver nanoparticles
subject: hydroxyapatite particles
subject: chitosan
subject: critical-size defect
subject: animal model
type: Text
identifier URI: http://hdl.handle.net/2429/90248