PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models
department or school: Applied Science, Faculty of
department or school: Medicine, Faculty of
department or school: Science, Faculty of
department or school: Biomedical Engineering, School of
department or school: Chemistry, Department of
department or school: Electrical and Computer Engineering, Department of
department or school: Pathology and Laboratory Medicine, Department of
data provider: DSpace
identifier citation: Micromachines 13 (10): 1573 (2022)
contributor: University of British Columbia. Centre for Blood Research
contributor: Djavad Mowafaghian Centre for Brain Health
creator: Cameron, Tiffany C.
creator: Randhawa, Avineet
creator: Grist, Samantha M.
creator: Bennet, Tanya
creator: Hua, Jessica
creator: Alde, Luis G.
creator: Caffrey, Tara M.
creator: Wellington, Cheryl L.
creator: Cheung, Karen C.
issued: 2024-12-18T16:25:32Z
issued: 2022-09-22
description: The PDMS-based microfluidic organ-on-chip platform represents an exciting paradigm that has enjoyed a rapid rise in popularity and adoption. A particularly promising element of this platform is its amenability to rapid manufacturing strategies, which can enable quick adaptations through iterative prototyping. These strategies, however, come with challenges; fluid flow, for example, a core principle of organs-on-chip and the physiology they aim to model, necessitates robust, leak-free channels for potentially long (multi-week) culture durations. In this report, we describe microfluidic chip fabrication methods and strategies that are aimed at overcoming these difficulties; we employ a subset of these strategies to a blood–brain-barrier-on-chip, with others applied to a small-airway-on-chip. Design approaches are detailed with considerations presented for readers. Results pertaining to fabrication parameters we aimed to improve (e.g., the thickness uniformity of molded PDMS), as well as illustrative results pertaining to the establishment of cell cultures using these methods will also be presented.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/89906
note: Citation: Cameron, T.C.; Randhawa,A.; Grist, S.M.; Bennet, T.; Hua, J.;Alde, L.G.; Caffrey, T.M.; Wellington,C.L.; Cheung, K.C. PDMSOrgan-On-Chip Design andFabrication: Strategies for ImprovingFluidic Integration and ChipRobustness of Rapidly PrototypedMicrofluidic In Vitro Models.Micromachines 2022, 13, 1573.https://doi.org/10.3390/mi13101573Academic Editor: Xudong CaoReceived: 3 August 2022Accepted: 15 September 2022Published: 22 September 2022Publisher’s Note: MDPI stays neutralwith regard to jurisdictional claims inpublished maps and institutional affil-iations.Copyright: © 2022 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).micromachinesArticlePDMS Organ-On-Chip Design and Fabrication: Strategies forImproving Fluidic Integration and Chip Robustness of RapidlyPrototyped Microfluidic In Vitro ModelsTiffany C. Cameron 1,2,*,†, Avineet Randhawa 1,2,*,†, Samantha M. Grist 1,2,3, Tanya Bennet 1,2, Jessica Hua 1,2,Luis G. Alde 2,4, Tara M. Caffrey 1,2 , Cheryl L. Wellington 5,6 and Karen C. Cheung 1,2,71 School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada2 Centre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, Canada3 Dream Photonics Inc., Vancouver, BC V6T 0A7, Canada4 Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada5 Department of Pathology and Laboratory Medicine, University of British Columbia,Vancouver, BC V6T 2B5, Canada6 Djavad Mowafaghian Centre for Brain Health, University of British Columbia,Vancouver, BC V6T 1Z3, Canada7 Department of Electrical and Computer Engineering, University of British Columbia,Vancouver, BC V6T 1Z4, Canada* Correspondence: tiffany.cameron321@gmail.com (T.C.C.); avineet.randhawa@protonmail.com oravineet.randhawa@ubc.ca (A.R.); Tel.: +1-(902)-598-8056…
has type: Article
is shown at: 10.14288/1.0447532
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/mi13101573
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: microfluidic
subject: organ-on-chip
subject: rapid prototyping
subject: cell culture
type: Text
identifier URI: http://hdl.handle.net/2429/89906