Optimization of Enzymatic Deproteination of Northern Shrimp (Pandalus borealis) Shell Chitin Using Commercial Proteases
department or school: Land and Food Systems, Faculty of
department or school: Non UBC
data provider: DSpace
identifier citation: Marine Drugs 22 (10): 445 (2024)
creator: Pohling, Julia
creator: Ramakrishnan, Vegneshwaran Vasudevan
creator: Hossain, Abul
creator: Trenholm, Sheila
creator: Dave, Deepika
issued: 2024-10-25T19:44:46Z
issued: 2024-09-28
description: Shrimp shells are a key source of chitin, commonly extracted through chemical methods, which may cause minor molecular damage. Nowadays, there is great interest in achieving close to zero protein content in crude chitin in order to use it for high-end markets. Therefore, this study optimized the enzymatic deproteination using two commercial proteases (SEB Pro FL100 and Sea-B Zyme L200) for effective and fast removal of residual protein from Northern shrimp (Pandalus borealis) shell chitin for the first time. The protein content was determined using both the Kjeldahl method and amino acid analysis using gas chromatography–mass spectrometry (GC-MS). The performance of papain (Sea B Zyme L200) was superior to fungal protease (SEB Pro FL100) for this application, and it achieved residual protein content of 2.01%, while the calculated optimum for the latter enzyme was 6.18%. A model was developed using 24 factorial design, and it was predicted that the lowest residual protein content using fungal protease and papain could be achieved at the following conditions: a pH of 4.2 and 7, and an enzyme concentration of 4 and 1.5%, respectively. Thus, the low-protein content obtained using enzymatic deproteination could be an alternative approach to the traditional methods, indicating their potential to produce premium-quality chitin.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/89542
note: Citation: Pohling, J.; Ramakrishnan,V.V.; Hossain, A.; Trenholm, S.; Dave,D. Optimization of EnzymaticDeproteination of Northern Shrimp(Pandalus borealis) Shell Chitin UsingCommercial Proteases. Mar. Drugs2024, 22, 445. https://doi.org/10.3390/md22100445Academic Editor: Paola LaurienzoReceived: 7 September 2024Revised: 24 September 2024Accepted: 26 September 2024Published: 28 September 2024Copyright: © 2024 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).marine drugs ArticleOptimization of Enzymatic Deproteination of Northern Shrimp(Pandalus borealis) Shell Chitin Using Commercial ProteasesJulia Pohling 1 , Vegneshwaran Vasudevan Ramakrishnan 1,2, Abul Hossain 2,3 , Sheila Trenholm 1and Deepika Dave 1,*1 Marine Institute, Center for Aquaculture and Seafood Development, Memorial University of Newfoundland,St. John’s, NL A1C5R3, Canada; julia.pohling@mi.mun.ca (J.P.); vvasudevanra@mun.ca (V.V.R.);sheila.trenholm@mi.mun.ca (S.T.)2 Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL A1C 5S7, Canada;abulh@mun.ca3 Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada* Correspondence: deepika.dave@mi.mun.caAbstract: Shrimp shells are a key source of chitin, commonly extracted through chemical methods,which may cause minor molecular damage. Nowadays, there is great interest in achieving close tozero protein content in crude chitin in order to use it for high-end markets. Therefore, this studyoptimized the enzymatic deproteination using two commercial proteases (SEB Pro FL100 and Sea-BZyme L200) for effective and fast removal of residual protein from Northern shrimp (Pandalus borealis)shell chitin for the first time. The protein content was determined using both the Kjeldahl method andamino acid analysis using gas chromatograp…
has type: Article
is shown at: 10.14288/1.0447144
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/md22100445
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: high-quality chitin
subject: commercial proteases
subject: Atlantic shrimp
subject: optimization
subject: waste utilization
type: Text
identifier URI: http://hdl.handle.net/2429/89542