Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species
department or school: Land and Food Systems, Faculty of
department or school: Non UBC
data provider: DSpace
identifier citation: Microorganisms 12 (4): 695 (2024)
creator: Bhandare, Sudhakar
creator: Lawal, Opeyemi U.
creator: Colavecchio, Anna
creator: Cadieux, Brigitte
creator: Zahirovich-Jovich, Yella
creator: Zhong, Zeyan
creator: Tompkins, Elizabeth
creator: Amitrano, Margot
creator: Kukavica-Ibrulj, Irena
creator: Boyle, Brian
creator: Wang, Siyun
creator: Levesque, Roger C.
creator: Delaquis, Pascal
creator: Danyluk, Michelle
creator: Goodridge, Lawrence
issued: 2024-05-09T21:24:53Z
issued: 2024-03-29
description: Bacteriophages (phages) are potential alternatives to chemical antimicrobials against pathogens of public health significance. Understanding the diversity and host specificity of phages is important for developing effective phage biocontrol approaches. Here, we assessed the host range, morphology, and genetic diversity of eight Salmonella enterica phages isolated from a wastewater treatment plant. The host range analysis revealed that six out of eight phages lysed more than 81% of the 43 Salmonella enterica isolates tested. The genomic sequences of all phages were determined. Whole-genome sequencing (WGS) data revealed that phage genome sizes ranged from 41 to 114 kb, with GC contents between 39.9 and 50.0%. Two of the phages SB13 and SB28 represent new species, Epseptimavirus SB13 and genera Macdonaldcampvirus, respectively, as designated by the International Committee for the Taxonomy of Viruses (ICTV) using genome-based taxonomic classification. One phage (SB18) belonged to the Myoviridae morphotype while the remaining phages belonged to the Siphoviridae morphotype. The gene content analyses showed that none of the phages possessed virulence, toxin, antibiotic resistance, type I–VI toxin–antitoxin modules, or lysogeny genes. Three (SB3, SB15, and SB18) out of the eight phages possessed tailspike proteins. Whole-genome-based phylogeny of the eight phages with their 113 homologs revealed three clusters A, B, and C and seven subclusters (A1, A2, A3, B1, B2, C1, and C2). While cluster C1 phages were predominantly isolated from animal sources, cluster B contained phages from both wastewater and animal sources. The broad host range of these phages highlights their potential use for controlling the presence of S. enterica in foods.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88266
note: Citation: Bhandare, S.; Lawal, O.U.;Colavecchio, A.; Cadieux, B.;Zahirovich-Jovich, Y.; Zhong, Z.;Tompkins, E.; Amitrano, M.;Kukavica-Ibrulj, I.; Boyle, B.; et al.Genomic and Phenotypic Analysis ofSalmonella enterica BacteriophagesIdentifies Two Novel Phage Species.Microorganisms 2024, 12, 695.https://doi.org/10.3390/microorganisms12040695Academic Editors: Lanying Zengand Junjie ZhangReceived: 8 March 2024Revised: 23 March 2024Accepted: 27 March 2024Published: 29 March 2024Copyright: © 2024 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).microorganismsArticleGenomic and Phenotypic Analysis of Salmonella entericaBacteriophages Identifies Two Novel Phage SpeciesSudhakar Bhandare 1,2,† , Opeyemi U. Lawal 3,† , Anna Colavecchio 1, Brigitte Cadieux 1, Yella Zahirovich-Jovich 1,Zeyan Zhong 1, Elizabeth Tompkins 1, Margot Amitrano 1 , Irena Kukavica-Ibrulj 4, Brian Boyle 4 , Siyun Wang 5 ,Roger C. Levesque 4 , Pascal Delaquis 6, Michelle Danyluk 7 and Lawrence Goodridge 3,*1 Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry,McGill University, Montreal, QC H9X 3V9, Canada; vetsudhakar@bristolalumni.org.uk orsudhakar.bhandare@nottingham.ac.uk (S.B.)2 School of Veterinary Medicine and Science, University of Nottingham, Nottingham LE12 5RD, UK3 Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph,Guelph, ON N1G 2W1, Canada; lawal@uoguelph.ca4 Institute for Integrative Systems Biology (IBIS), Laval University, Québec, QC G1V 0A6, Canada;rclevesq@ibis.ulaval.ca (R.C.L.)5 Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC V6T 1Z4, Canada;siyun.wang@ubc.ca6 Agriculture and Agri-Food Canada, Summerland, BC V0H 1Z0, Canada7 Food Science and Human Nutrition Department, University of Florida, Gainesville…
has type: Article
is shown at: 10.14288/1.0442419
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: Multidisciplinary Digital Publishing Institute
publisher DOI: 10.3390/microorganisms12040695
rights: CC BY 4.0
rights URI: https://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
scholar level: Researcher
subject: Salmonella
subject: bacteriophage
subject: diversity
subject: host specificity
subject: comparative phylogenomics
subject: food safety
type: Text
identifier URI: http://hdl.handle.net/2429/88266