Immunoglobulin recognition of fecal bacteria in stunted and non-stunted children: findings from the Afribiota study
department or school: Medicine, Faculty of
department or school: Science, Faculty of
department or school: Non UBC
department or school: Biochemistry and Molecular Biology, Department of
department or school: Microbiology and Immunology, Department of
department or school: Population and Public Health (SPPH), School of
data provider: DSpace
identifier citation: Microbiome. 2020 Jul 27;8(1):113
contributor: Michael Smith Laboratories
rights copyright: The Author(s)
creator: Huus, Kelsey E.
creator: Rodriguez-Pozo, André
creator: Kapel, Nathalie
creator: Nestoret, Alison
creator: Habib, Azimdine
creator: Dede, Michel
creator: Manges, Amee R.
creator: Collard, Jean-Marc
creator: Sansonetti, Philippe J.
creator: Vonaesch, Pascale
creator: Finlay, B. Brett, 1959-
issued: 2020-07-31T22:01:43Z
issued: 2020-07-27
description: Background Child undernutrition is a global health issue that is associated with poor sanitation and an altered intestinal microbiota. Immunoglobulin (Ig) A mediates host-microbial homeostasis in the intestine, and acutely undernourished children have been shown to have altered IgA recognition of the fecal microbiota. We sought to determine whether chronic undernutrition (stunting) or intestinal inflammation were associated with antibody recognition of the microbiota using two geographically distinct populations from the Afribiota project. Fecal bacteria from 200 children between 2 and 5 years old in Antananarivo, Madagascar, and Bangui, Central African Republic (CAR), were sorted into IgA-positive (IgA+) and IgA-negative (IgA−) populations by flow cytometry and subsequently characterized by 16S rRNA gene sequencing to determine IgA-bacterial targeting. We additionally measured IgG+ fecal bacteria by flow cytometry in a subset of 75 children. Results Stunted children (height-for-age z-score ≤ −2) had a greater proportion of IgA+ bacteria in the fecal microbiota compared to non-stunted controls. This trend was consistent in both countries, despite the higher overall IgA-targeting of the microbiota in Madagascar, but lost significance in each country individually. Two of the most highly IgA-recognized bacteria regardless of nutritional status were Campylobacter (in CAR) and Haemophilus (in both countries), both of which were previously shown to be more abundant in stunted children; however, there was no association between IgA-targeting of these bacteria and either stunting or inflammatory markers. IgG-bound intestinal bacteria were rare in both stunted and non-stunted children, similar to levels observed in healthy populations. Conclusions Undernourished children carry a high load of intestinal pathogens and pathobionts. Our data suggest that stunted children have a greater proportion of IgA-recognized fecal bacteria. We moreover identify two putative pathobionts, Ha…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/75352
note: RESEARCH Open AccessImmunoglobulin recognition of fecalbacteria in stunted and non-stuntedchildren: findings from the Afribiota studyKelsey E. Huus1,2, André Rodriguez-Pozo3, Nathalie Kapel4, Alison Nestoret4, Azimdine Habib5, Michel Dede6,Amee Manges7, Jean-Marc Collard8, Philippe J. Sansonetti3,9†, Pascale Vonaesch3,10†, B. Brett Finlay1,2,11*† and forthe Afribiota InvestigatorsAbstractBackground: Child undernutrition is a global health issue that is associated with poor sanitation and an alteredintestinal microbiota. Immunoglobulin (Ig) A mediates host-microbial homeostasis in the intestine, and acutelyundernourished children have been shown to have altered IgA recognition of the fecal microbiota. We sought todetermine whether chronic undernutrition (stunting) or intestinal inflammation were associated with antibodyrecognition of the microbiota using two geographically distinct populations from the Afribiota project. Fecalbacteria from 200 children between 2 and 5 years old in Antananarivo, Madagascar, and Bangui, Central AfricanRepublic (CAR), were sorted into IgA-positive (IgA+) and IgA-negative (IgA−) populations by flow cytometry andsubsequently characterized by 16S rRNA gene sequencing to determine IgA-bacterial targeting. We additionallymeasured IgG+ fecal bacteria by flow cytometry in a subset of 75 children.Results: Stunted children (height-for-age z-score ≤ −2) had a greater proportion of IgA+ bacteria in the fecalmicrobiota compared to non-stunted controls. This trend was consistent in both countries, despite the higheroverall IgA-targeting of the microbiota in Madagascar, but lost significance in each country individually. Two of themost highly IgA-recognized bacteria regardless of nutritional status were Campylobacter (in CAR) and Haemophilus(in both countries), both of which were previously shown to be more abundant in stunted children; however, therewas no association between IgA-targeting of these bacteria and either stunting or inflammatory marker…
has type: Article
is shown at: 10.14288/1.0392619
language: eng
peer review status: Reviewed
provider: Vancouver : University of British Columbia Library
publisher: BioMed Central
publisher DOI: 10.1186/s40168-020-00890-1
rights: Attribution 4.0 International (CC BY 4.0)
rights URI: http://creativecommons.org/licenses/by/4.0/
scholar level: Faculty
subject: Microbiome
subject: Immunoglobulin A
subject: Immunoglobulin G
subject: Undernutrition
subject: Stunting
subject: Environmental Enteric dysfunction
subject: Sub-Saharan Africa
type: Text
identifier URI: http://hdl.handle.net/2429/75352