Development of microfluidic platforms for low-cost and high-precision droplet generation and quantification of agglutination assays
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Zia, Abdul Basit
issued: 2025-01-16T22:06:05Z
issued: 2025
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: This dissertation explores the development and calibration of a cost-effective, automated microfluidic droplet-on-demand generation system by adapting fused deposition modeling (FDM) 3D printer to overcome the high costs and complexities traditionally associated with droplet microfluidics. The developed systems can move the inlet of the microfluidic system in cartesian coordinates and generate droplets from various analytes, termed automated dynamic inlet microfluidic systems (ADIM systems). A 3D printer (Prusa Mini+) is adapted with novel 3D printed modules into a droplet-on-demand system that generates combinatorial droplets from a standard 96-well plate in dual-phase microfluidics. The calibration methodology developed would allow any FDM printer to create monodisperse droplets (coefficient of variance (CV%) < 5%) from well plates or vials of any geometry submerged in oil. The system maintains precision across various volumes while maintaining a C.V. range of 0.81% to 3.61%. The cost of the system developed is 70% less than commercially available droplet generation packages. The developed ADIM system is used to generate trains of droplets of analyte and quantified across a broad concentration range (0.0128 μg/mL to 5000 μg/mL). The analysis methodology utilizes MATLAB for video frame extraction and processing, and develops multiple metrics for the droplet images, each providing a different insight. This image analysis approach successfully demonstrates the system’s ability to differentiate between agglutinated and non-agglutinated droplets across the entire range of concentrations. Moreover, the system can classify the agglutination into three regions (Band 1: 0.0128 to 0.32 μg/mL, Band 2: 1.6 to 40 μg/mL, and Band 3: 200 to 5000 μg/mL) with a 97.50% accuracy for image analysis. Building upon our initial model, the second ADIM system simplifies calibration methodology with an inverted inlet to produce mono-disperse droplets (CV% < 2% for a train of 100 droplets).…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/90136
note: Development of Microfluidic Platformsfor Low-Cost and High Precision DropletGeneration and Quantification ofAgglutination AssaysbyAbdul Basit ZiaB.Sc., Ghulam Ishaq Khan Institute, Pakistan, 2011M.Eng., University of Malaya, 2014A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OFTHE REQUIREMENTS FOR THE DEGREE OFDOCTOR OF PHILOSOPHYinThe College of Graduate Studies(Electrical Engineering)THE UNIVERSITY OF BRITISH COLUMBIA(Okanagan)January 2025© Abdul Basit Zia, 2025The following individuals certify that they have read, and recommend to theCollege of Graduate Studies for acceptance, a dissertation entitled:Development of Microfluidic Platforms for Low-Cost and HighPrecision Droplet Generation and Quantification of Aggluti-nation Assayssubmitted by Abdul Basit Zia in partial fulfilment of the requirements ofthe degree of Doctor of PhilosophyDr. Ian G. Foulds, School of EngineeringSupervisorDr. Mohammad Hossein Zarifi, School of EngineeringSupervisory Committee MemberDr. Christoper Collier, School of EngineeringSupervisory Committee MemberDr. Jennifer Frattolin, School of EngineeringUniversity ExaminerDr. Xinyu Liu, University of TorontoExternal ExamineriiAbstractThis dissertation explores the development and calibration of a cost-effective,automated microfluidic droplet-on-demand generation system by adaptingfused deposition modeling (FDM) 3D printer to overcome the high costsand complexities traditionally associated with droplet microfluidics. Thedeveloped systems can move the inlet of the microfluidic system in cartesiancoordinates and generate droplets from various analytes, termed automateddynamic inlet microfluidic systems (ADIM systems).A 3D printer (Prusa Mini+) is adapted with novel 3D printed modules intoa droplet-on-demand system that generates combinatorial droplets from astandard 96-well plate in dual-phase microfluidics. The calibration method-ology developed would allow any FDM printer to create monodisperse droplets(coefficient of variance (CV%) < 5%) fr…
has type: Thesis/Dissertation
date issued: 2025-05
is shown at: 10.14288/1.0447761
language: eng
degree discipline: Electrical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Foulds, Ian G.
type: Text
type: Moving Image
type: Still Image
identifier URI: http://hdl.handle.net/2429/90136