The effect of abiotic and biotic plant stress on Phaeomoniella chlamydospora abundance and disease development in young grapevines
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Biology, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Hrycan, Jared
issued: 2024-07-17T16:11:36Z
issued: 2024
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Young vine decline (YVD) fungal pathogens cause internal necrosis, leading to stunted growth and eventual plant mortality within the first few years after planting. Canada’s fast-growing grape and wine industry depends on the import of nursery propagated material but the health status of ready-to-plant nursery grapevines in Canada regarding YVD fungi is unclear. A nursery survey was conducted to investigate the presence or absence and quantity of the most prevalent YVD pathogens reported in other countries. 99% of plants contained at least one YVD pathogen, and overall, a mean of 3 YVD fungal species were found in each grapevine. Pathogen abundance for each fungus in single plants was conducted using droplet digital PCR. Results showed fungal abundance to vary widely between nurseries, grapevines, and different parts of the same grapevines. Additionally, several YVD pathogens were isolated that had not previously been reported in Canada, showing that nursery material can be the source or new fungal pathogens in Canadian vineyards. Phaeomoniella chlamydospora (Pc), one of the main agents of the YVD Petri disease, was found in up to 80% of ready-to-plant nursery grapevines. The presence of Pc in asymptomatic nursery grapevines has led to the hypothesis that it may be a latent pathogen, transitioning from an endophyte to a pathogen under plant stress. Two greenhouse and four field experiments were conducted to determine the effect of water stress, heat stress, j-rooting, and ring nematode infestation on Pc abundance and disease development in young grapevines. Arbuscular mycorrhizal (AM) fungi were inoculated in the greenhouse to investigate whether a reduction in plant stress due to AM fungal colonization would reduce Pc abundance. In the greenhouse, a combination of water stress and AM fungal colonization increased Pc abundance. Young vine decline like symptom development was observed in the field and higher mortality occurred in grapevines subjected to the 50% water…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88650
note: THE EFFECT OF ABIOTIC AND BIOTIC PLANT STRESS ON PHAEOMONIELLA CHLAMYDOSPORA ABUNDANCE AND DISEASE DEVELOPMENT IN YOUNG GRAPEVINES by Jared Hrycan B.Sc. University of British Columbia Okanagan, 2018 A THESIS SUBMITTED IN PARTIAL FULLFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) July 2024 © Jared Hrycan, 2024 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: THE EFFECT OF ABIOTIC AND BIOTIC PLANT STRESS ON PHAEOMONIELLA CHLAMYDOSPORA ABUNDANCE AND DISEASE DEVELOPMENT IN YOUNG GRAPEVINES submitted by Jared Hrycan in partial fulfillment of the requirements of the degree of Doctor of Philosophy Dr. Miranda Hart, Irving K. Barber School of Arts and Sciences Supervisor Dr. José Ramón Úrbez-Torres, Agriculture and Agri-Food Canada, Summerland Co-Supervisor Dr. Patricia Bowen, Agriculture and Agri-Food Canada, Summerland Supervisory Committee Member Dr. Thomas Forge, Agriculture and Agri-Food Canada, Summerland Supervisory Committee Member Dr. Louise Nelson, Irving K. Barber School of Arts and Sciences Supervisory Committee Member Dr. Gurcharn Singh Brar, University of British Columbia University Examiner Dr. Philippe E. Rolshausen, University of California, Riverside External Examiner iii Abstract Young vine decline (YVD) fungal pathogens cause internal necrosis, leading to stunted growth and eventual plant mortality within the first few years after planting. Canada’s fast-growing grape and wine industry depends on the import of nursery propagated material but the health status of ready-to-plant nursery grapevines in Canada regarding YVD fungi is unclear. A nursery survey was conducted to investigate the presence or absence and quantity of the most prevalent YVD pathogens reported in other countries. 99% of plants contained at least one YVD pathogen, and over…
has type: Thesis/Dissertation
date issued: 2024-09
is shown at: 10.14288/1.0444166
language: eng
degree discipline: Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Hart, Miranda
contributor: Urbez-Torres, Jose Ramon
type: Text
type: Dataset
identifier URI: http://hdl.handle.net/2429/88650