Deformability based cell sorting enabling quality control of donated red blood cells
department or school: Medicine, Faculty of
department or school: Pathology and Laboratory Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Islamzada, Emel
issued: 2024-05-03T19:39:40Z
issued: 2024
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Red blood cell (RBC) transfusions are a critical component of patient care, with around 1.2 million units utilized annually in Canada alone. Patients with acute blood loss require temporary RBC transfusions until their erythropoiesis restores RBC levels. Those with chronic disorders, such as hemoglobinopathies and bone marrow cancers, rely on transfused RBCs for entire RBC circulatory lifespan. For these chronic recipients, selecting long-circulating RBC units could minimize transfusion-associated morbidity like iron overload and lung damage, yet established methods to identify these units are lacking. This dissertation studied the potential to use RBC deformability as a surrogate marker for the transfused RBC circulatory clearance time. Deformability is vital for RBC function and has been considered a physical biomarker of circulation time in transfusion recipients. Our team has developed a unique microfluidic ratchet device to analyze RBC deformability. This device operates by directing individual RBCs to deform through a series of micrometer-scale constrictions much smaller than RBC diameter. A consistent force gradient is applied to each cell as it deforms through constrictions, enabling measurement of RBC deformability with high sensitivity and repeatability. Using the microfluidic ratchet device, we show that RBC deformability varies across healthy donors, but is consistent for each donor over multiple donations. Furthermore, RBC deformability is preserved during the standard 42-day cold storage in blood bags but degrades significantly beyond this timeframe. Additionally, deformability can be estimated by sampling from the blood bag tubing segment instead of puncturing the bag. Finally, we use a mouse transfusion model to investigate whether low-deformability RBCs are preferentially cleared from circulation. We show the rigid fraction of transfused RBCs are rapidly cleared, while transfused RBCs remaining in circulation had a deformability profile closely matc…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/88207
note: DEFORMABILITY BASED CELL SORTING ENABLING QUALITY CONTROL OF DONATED RED BLOOD CELLS by Emel Islamzada B.Sc., The University of British Columbia, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in The Faculty of Graduate and Postdoctoral Studies (Pathology and Laboratory Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2024 © Emel Islamzada, 2024 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Deformability based cell sorting enabling quality control of donated red blood cells submitted by Emel Islamzada in partial fulfilment of the requirements for the degree of Doctor of Philosophy In Pathology and Laboratory Medicine Examining Committee: Dr. Hongshen Ma, Mechanical Engineering, UBC Supervisor Dr. Mark D. Scott, Pathology and Laboratory Medicine, UBC Co-supervisor Dr. Cedric J. Carter, Pathology and Laboratory Medicine, UBC Supervisory Committee Member Dr. James Feng, Chemical and Biological Engineering, UBC University Examiner Dr. Edward Conway, Medicine, UBC University Examiner Additional Supervisory Committee Members: Dr. Karen Cheung, Electrical and Computer Engineering, UBC Supervisory Committee Member iii Abstract Red blood cell (RBC) transfusions are a critical component of patient care, with around 1.2 million units utilized annually in Canada alone. Patients with acute blood loss require temporary RBC transfusions until their erythropoiesis restores RBC levels. Those with chronic disorders, such as hemoglobinopathies and bone marrow cancers, rely on transfused RBCs for entire RBC circulatory lifespan. For these chronic recipients, selecting long-circulating RBC units could minimize transfusion-associated morbidity like iron overload and lung damage, yet established methods to identify these units are lacking. This dissertation studied the potential to use RBC deformab…
has type: Thesis/Dissertation
date issued: 2024-11
is shown at: 10.14288/1.0442264
language: eng
degree discipline: Pathology and Laboratory Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Ma, Hongshen
contributor: Scott, Mark Douglas
type: Text
identifier URI: http://hdl.handle.net/2429/88207