Exploring the cannabis microbiome to develop safe and sustainable bio-fungicide
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Biology, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Dumigan, Christopher Robert
issued: 2024-04-10T17:27:27Z
issued: 2024
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Cannabis plants are hosts to a variety of fungal pathogens that have limited tools for control. Studying the microbiome of other crops shows that microorganisms can play an integral role in plant health by antagonizing pathogens, promoting plant vigor, and creating a functioning community or a “healthy” microbiome. Furthermore, plant-associated microorganisms can be passed down plant generations to germinating seedlings by their mother plant and vectored by seed. These can be both pathogenic and beneficial to their host plant. This thesis investigates the microbiome of Cannabis plants and the activities that both beneficial and pathogenic microorganisms have on this crop. Investigating 15 genotypes of Cannabis grown to seed across western Canada showed both hemp and marijuana inherit a closely related group of bioactive and anti-fungal endophytic bacilli. 13 of these isolates were able to antagonize known fungal pathogens of Cannabis that were also seed-borne, suggesting co-evolution and potential symbiosis between Cannabis and this class of bacteria. Mature Cannabis plants are infected by a growing number of fungal diseases. Documented here is the first report of Berkeleyomyces rouxiae infecting drug-type Cannabis causing black root-rot. This fungal pathogen was isolated from an aeroponics facility in Cranbrook BC and was not identified due to a lack of genomic resources on this fungal species. Here the first whole genome sequence is provided using hybrid genome sequencing and assembly. Finally, this thesis investigates the relative contributions of both seed and soil bacteria in shaping the endosphere communities in germinating Cannabis seedlings. A multifactorial metagenomic experiment was performed using three genotypes of Cannabis with two soil types, both of which were treated and untreated with autoclave sterilization. Cannabis genotype, soil type, and soil sterilization all play a role in structuring the endophyte communities of germinating Cannabis seedling…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/87729
note: Exploring the Cannabis Microbiome to Develop Safe and Sustainable Bio-Fungicides by Christopher Robert Dumigan MSc, University of Guelph 2018 BSc, Western University 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) March 2024 © Christopher Dumigan, 2024 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Exploring the Cannabis Microbiome to Develop Safe and Sustainable Bio-Fungicides submitted by Christopher Robert Dumigan in partial fulfillment of the requirements of the degree of Doctor of Philosophy. Dr. Michael K Deyholos, Biology, Irving K. Barber Faculty of Science Supervisor Dr. Miranda Hart, Biology, Irving K. Barber Faculty of Science Supervisory Committee Member Dr. Deanna Gibson, Biology, Irving K. Barber Faculty of Science Supervisory Committee Member Dr. Susan Murch, Chemistry, Irving K. Barber Faculty of Science Supervisory Committee Member Dr. Kirsten Wolthers, Chemistry, Irving K. Barber Faculty of Science University Examiner Dr. Zamir Punja, Department of Biological Sciences, Simon Fraser University External Examiner iii Abstract Cannabis plants are hosts to a variety of fungal pathogens that have limited tools for control. Studying the microbiome of other crops shows that microorganisms can play an integral role in plant health by antagonizing pathogens, promoting plant vigor, and creating a functioning community or a “healthy” microbiome. Furthermore, plant-associated microorganisms can be passed down plant generations to germinating seedlings by their mother plant and vectored by seed. These can be both pathogenic and beneficial to their host plant. This thesis investigates the microbiome of Cannabis plants and the activities that both beneficial and pathogenic microorganisms have on this crop. Inves…
has type: Thesis/Dissertation
date issued: 2024-05
is shown at: 10.14288/1.0441272
language: eng
degree discipline: Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Deyholos, Michael
type: Text
type: Dataset
identifier URI: http://hdl.handle.net/2429/87729