Development and characterization of photoluminescent materials towards applications of concentric Fӧrster resonance energy transfer probes in bioanalysis
department or school: Science, Faculty of
department or school: Chemistry, Department of
data provider: DSpace
degree campus: UBCV
creator: Tsai, Hsin-Yun
issued: 2024-06-30T07:00:00Z
issued: 2023
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Förster resonance energy transfer (FRET) is widely used for developing fluorescent probes for bioanalysis and imaging. Typically, multiplexed sensing with FRET involves the deployment of multiple probes. In contrast, concentric FRET (cFRET) is a design motif that enables multiplexed sensing with a single probe. Prior to this thesis, cFRET probes had only been developed around metal chalcogenide quantum dots (QDs), which may limit the scope of biological applications. This thesis focuses on expanding the materials palette of cFRET, with the goal of exploring and evaluating new capabilities and enhancing its suitability for more diverse applications. To avoid metal chalcogenide QDs, an alternative cFRET probe was developed using poly(amidoamine) dendrimers labelled with a luminescent terbium complex (Tb). This material was functionalized with three types of dye-labelled oligonucleotides to establish a network of FRET pathways. The new cFRET probes were characterized, and a time-gated multiplexed assay for DNA targets was demonstrated. The time-gating oﬀered also enabled the rejection of background autoﬂuorescence from serum. A derivative FRET probe was compatible with a smartphone-based time-gated imaging device, suggesting potential applications in point-of-care diagnostics. Silicon QDs, also called silicon nanocrystals (SiNCs), are another potential alternative to metal chalcogenide QDs. The size and PL properties of SiNCs were characterized via spectroelectrophoresis and time-resolved and time-gated spectroscopies. SiNCs were then paired as FRET donors with a series of dark quenchers or a fluorescent dye for systematically studying energy transfer. The polydispersity of the SiNCs and resulting heterogeneity of PL properties made these materials non-ideal for cFRET. Lastly, metal chalcogenide QD-based cFRET probes were structurally integrated with a hydrogel toward sensing cell-secreted protease activity. The hydrogel mimics the extracellular matrix to provide a mor…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/85174
note: DEVELOPMENT AND CHARACTERIZATION OF PHOTOLUMINESCENT MATERIALS TOWARDS APPLICATIONS OF CONCENTRIC FӦRSTER RESONANCE ENERGY TRANSFER PROBES IN BIOANALYSIS by Hsin-Yun Tsai A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Chemistry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) June 2023 © Hsin-Yun Tsai, 2023 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Development and Characterization of Photoluminescent Materials Towards Applications of Concentric Fӧrster Resonance Energy Transfer Probes in Bioanalysis submitted by Hsin-Yun Tsai in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry Examining Committee: W. Russ Algar, Associate Professor, Chemistry, UBC Supervisor Jayachandran Kizhakkedathu, Professor, Pathology and Laboratory Medicine, UBC Supervisory Committee Member Michael Wolf, Professor, Chemistry, UBC University Examiner Urs Hafeli, Professor, Pharmaceutical Sciences, UBC University Examiner Additional Supervisory Committee Members: Roman Krems, Professor, Chemistry, UBC Supervisory Committee Member Karen Cheung, Professor, Electrical and Computer Engineering, UBC Supervisory Committee Member iii Abstract Förster resonance energy transfer (FRET) is widely used for developing fluorescent probes for bioanalysis and imaging. Typically, multiplexed sensing with FRET involves the deployment of multiple probes. In contrast, concentric FRET (cFRET) is a design motif that enables multiplexed sensing with a single probe. Prior to this thesis, cFRET probes had only been developed around metal chalcogenide quantum dots (QDs), which may limit the scope of biological applications. This thesis focuses on expanding the materials palette of cFRET, with the goal of exploring and evaluating new capabilities and enh…
has type: Thesis/Dissertation
date issued: 2023-11
is shown at: 10.14288/1.0433816
language: eng
degree discipline: Chemistry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Algar, W. Russ
type: Text
identifier URI: http://hdl.handle.net/2429/85174