Evaluating the anti-bacterial and wound healing promoting effects of PVP/PVP-I containing liquid scaffold in vitro and in vivo
department or school: Medicine, Faculty of
department or school: Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Abbasidezfouli, Samin
issued: 2023-06-28T08:05:23Z
issued: 2023
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Background: Based on the WHO report in 2018, 11 million burn injuries occur worldwide annually, 180,000 of which are lethal. Many studies over the past few years have demonstrated that infection and delayed wound healing are responsible for 42%–65% mortality in burn victims. Hence, the development and application of an antibacterial nutritional liquid scaffold in large burn injuries could reduce the infection rate, accelerate wound healing, and ultimately, save lives. MeshFill is a nutritional liquid scaffold found to improve wound healing and esthetic outcomes upon application in a split-thickness meshed skin graft (STMSG) in a porcine model. To expand the efficacy of MeshFill and address the prevention of infections due to wound contamination, our group added antimicrobial properties by replacing polyvinyl alcohol (PVA), a component in the MeshFill complex, with Povidone-iodine (PVP-I). Unlike silver and antibiotics, low concentrations of iodine are not toxic to human cells, and there is no potential for bacterial resistance. Experimental approach: We assessed the antibacterial effect of various ratios of PVP-I/PVP scaffoderm on both gram-positive and gram-negative bacteria during the 4- and 24-hour time points. The cytotoxicity of the three best ratios of PVP-I/PVP scaffoderm was assessed in vitro, while the wound healing effects in terms of antibacterial activity were then evaluated in vivo using relevant rat models. Result: In terms of antibacterial activity, 150% and 200% PVP-I/PVP scaffoderm was shown to kill gram-positive and gram-negative species most effectively in comparison with other groups. Moreover, 100% and 150% PVP-I/PVP scaffoderm showed the best healing results in vivo and did not delay the wound healing process. Conclusion: Scaffoderm containing 150% PVP-I/PVP is most efficacious in terms of antibacterial activity in full-thickness wounds and the promotion of wound healing. The incorporation of PVP-I into MeshFill may be an effective method to pr…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/85166
note: Evaluating the anti-bacterial and wound healing promoting effects of PVP/PVP-I containing liquid scaffold in vitro and in vivo. by Samin Abbasidezfouli A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) June 2023 © Samin Abbasidezfouli, 2023 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Evaluating the anti-bacterial and wound healing promoting effects of PVP/PVP-I containing liquid scaffold in vitro and in vivo. submitted by Samin Abbasidezfouli in partial fulfillment of the requirements for the degree of Master of Science in Experimental Medicine Examining Committee: Dirk Lange, Associate Professor, Department of Urologic Sciences, UBC Supervisor David Granville, Professor, Department of Pathology and Laboratory Medicine, UBC Supervisory Committee Member Erin Brown, Clinical Professor, Department of Surgery, UBC Additional Examiner iii Abstract Background: Based on the WHO report in 2018, 11 million burn injuries occur worldwide annually, 180,000 of which are lethal. Many studies over the past few years have demonstrated that infection and delayed wound healing are responsible for 42%–65% mortality in burn victims. Hence, the development and application of an antibacterial nutritional liquid scaffold in large burn injuries could reduce the infection rate, accelerate wound healing, and ultimately, save lives. MeshFill is a nutritional liquid scaffold found to improve wound healing and esthetic outcomes upon application in a split-thickness meshed skin graft (STMSG) in a porcine model. To expand the efficacy of MeshFill and address the prevention of infections due to wound contamination, our group added antimicrobial properties by replacing polyvinyl alcohol (PVA), a component in the Mes…
has type: Thesis/Dissertation
date issued: 2023-11
is shown at: 10.14288/1.0433755
language: eng
degree discipline: Experimental Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Lange, Dirk (Microbiologist)
type: Text
identifier URI: http://hdl.handle.net/2429/85166