Comparing the sucrose synthase promoters in Arabidopsis thaliana and hybrid poplar
department or school: Forestry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Muirragui, Emilia
issued: 2023-04-21T17:02:43Z
issued: 2023
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Sucrose synthase is a glycosyl transferase enzyme responsible for catalyzing the reversible cleavage of sucrose into fructose and UDP-glucose in terrestrial plants. This role in sucrose breakdown has been associated with determining sink strength, modulating phloem loading, and possibly participating in cellulose and starch biosynthesis. In Arabidopsis thaliana, all six SuSy isoforms have been observed to localize to vascular tissues other than the xylem, a major site of cellulose deposition. However, it is highly possible that SuSy plays a more significant role in cellulose biosynthesis in other plant species, such as the aspen family (Populus spp.), where several isoforms have been identified to localize preferentially to the mature xylem. Previous studies have demonstrated that promoters play essential roles in transcriptional regulation, and that the regulatory elements found in their distal and proximal regions could be fundamental to regulating spatiotemporal gene expression. As such, the primary objective of this thesis was to identify differences in the spatiotemporal localization of SuSy in A. thaliana and P. trichocarpa, by assessing the putative contribution of the promoter regions to the functional role(s) of SuSy in plant growth and development. This was investigated by first identifying essential motifs via a bioinformatic analysis of PtSuSy1, PtSuSy2, AtSuSy1, AtSuSy4 promoter sequences, and then comparing enzyme localization via histochemical staining and live-cell imaging across the vascular tissues of A. thaliana and P. trichocarpa when driven by the same promoter (PtSUS1p). Two SNBE-like motifs were identified in the distal region of the poplar SuSy promoters, and given their absence from the Arabidopsis orthologs, were selected for further analysis via promoter truncation experiments. These, along with other GUS transformants, had a sequencing error which caused a frameshift mutation preventing verified transgenic plants from producing visible GU…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/84400
note: COMPARING SUCROSE SYNTHASE PROMOTERS IN ARABIDOPSIS THALIANA AND HYBRID POPLAR by Emilia MUIRRAGUI B.Sc., University of British Columbia, 2019 A THESIS SUBMITTED IN PARTIAL FULLFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Forestry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2023  Emilia Muirragui, 2023 iiThe following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Comparing sucrose synthase promoters in Arabidopsis thaliana and hybrid poplar submitted by Emilia Muirragui in partial fulfilment of the requirements for the degree of Master of Science in Forestry Examining Committee: Dr. Shawn D. Mansfield, Professor, Wood Science, UBC Supervisor Dr. Lacey A. Samuels, Professor, Botany, UBC Supervisory Committee Member Dr. Abel Rosado, Associate Professor, Botany, UBC Supervisory Committee Member Dr. Sean Crowe, Associate Professor, Microbiology and Immunology, UBC Additional Examiner iiiAbstract Sucrose synthase is a glycosyl transferase enzyme responsible for catalyzing the reversible cleavage of sucrose into fructose and UDP-glucose in terrestrial plants. This role in sucrose breakdown has been associated with determining sink strength, modulating phloem loading, and possibly participating in cellulose and starch biosynthesis. In Arabidopsis thaliana, all six SuSy isoforms have been observed to localize to vascular tissues other than the xylem, a major site of cellulose deposition. However, it is highly possible that SuSy plays a more significant role in cellulose biosynthesis in other plant species, such as the aspen family (Populus spp.), where several isoforms have been identified to localize preferentially to the mature xylem. Previous studies have demonstrated that promoters play essential roles in transcriptional regulation, and that the regulatory elements found in their distal and p…
has type: Thesis/Dissertation
date issued: 2023-05
is shown at: 10.14288/1.0431369
language: eng
degree discipline: Forestry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Mansfield, Shawn D., 1969-
type: Text
identifier URI: http://hdl.handle.net/2429/84400