How gut microbiome correlates with oxalate, butyrate and kidney stones formation
department or school: Medicine, Faculty of
department or school: Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Felix, Demian Ferreira
issued: 2022-11-28T23:58:43Z
issued: 2022
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Background: Kidney stone disease (KSD) is a worldwide metabolic disorder, that affects about 12% of the world population at some stage of life, representing a financial burden for health systems. The prevalence of urolithiasis is increasing in the past decades, despite progress in the surgical procedures to remove the stones, there is a recurrence rate after the intervention, and there is no definitive cure for it. In the past decades, the role of intestinal microbiota regarding KSD pathology has been gradually unravelled. Oxalate-degrading bacteria and short-chain fatty acid (SCFA) producing microorganisms have been shown to have important roles in crystal formation through direct calcium oxalate breakdown or as inducers of immune responses capable of inhibiting crystal formation in the kidneys. Experimental approach: In order to test the properties of prebiotics in stone formation physiology, we used an in vivo mouse model administering four different formulated diets. A control diet, a sodium oxalate diet to stimulate stone formation, an inulin diet to stimulate SCFA-producing microorganisms, and a tributyrin diet to act as a direct source of butyrate, a specific type of SCFA that we have previously shown to be lacking in kidney stone patients. Results: Results indicated that the sodium oxalate diet reduced the overall mice microbial alpha diversity and the variability of bacterial groups responsible for SCFA production. It also increased oxalate concentrations in the urine and caused crystal formation in the kidney. Furthermore, an inulin diet was able to promote the proliferation of SCFA-related microbiota and SCFA concentrations in the gut, which resulted in reduced oxalate amounts in the urine, therefore, contributing to reducing the risk of stone formation. Tributyrin supplementation did not promote any significant effect. Conclusions: This study corroborates previous research indicating that dietary supplements like inulin are potential prebiotic candidates…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/83317
note: HOW GUT MICROBIOME CORRELATES WITH OXALATE, BUTYRATE AND KIDNEY STONES FORMATION by Demian Ferreira Felix B.Sc., The Pontifical Catholic University of Minas Gerais, 2004 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) November 2022 © Demian Ferreira Felix, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: How gut microbiome correlates with oxalate, butyrate and kidney stones formation submitted by Demian Ferreira Felix in partial fulfillment of the requirements for the degree of Master of Science in Experimental Medicine Examining Committee: Dr. Dirk Lange, Associate Professor, Urologic Sciences, UBC Supervisor Dr. Ben Chew, Associate Professor, Urologic Sciences, UBC Supervisory Committee Member Dr. Genelle Healey, Posdoctoral Research Fellow, Pediatrics, BC Children's Hospital, UBC Supervisory Committee Member Dr. Gregory E Tasian, Pediatric Urologist, Urology, Children's Hospital of Philadelphia Additional Examiner iii Abstract Background: Kidney stone disease (KSD) is a worldwide metabolic disorder, affecting about 12% of the world population at some stage of their life, representing a financial burden for health systems. The prevalence of urolithiasis is increasing in the past decades, despite progress in the surgical procedures to remove the stones, there is a recurrence rate after intervention, and there is not a definitive cure for it. In the past decades, the role of intestinal microbiota regarding KSD pathology has been gradually unraveled. Oxalate degrading bacteria and short-chain fatty acid (SCFA) producing microorganisms have shown to have important roles in crystal formation through direct calcium oxalate break down or as inducers of immune responses capable of inhibi…
has type: Thesis/Dissertation
date issued: 2023-05
is shown at: 10.14288/1.0422190
language: eng
degree discipline: Experimental Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Lange, Dirk (Microbiologist)
type: Text
identifier URI: http://hdl.handle.net/2429/83317