Short-term resistance exercise training attenuates muscle damage and alters transcription of muscle extracellular matrix regulators in response to 300 eccentric contractions in young adults
department or school: Education, Faculty of
department or school: Kinesiology, School of
data provider: DSpace
degree campus: UBCV
creator: Li , Ching Wah Donna
issued: 2022-11-21T21:58:57Z
issued: 2023
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: A maximal eccentric (ECC) bout can confer protection by reducing exercise-induced muscle damage (EIMD) following a subsequent ECC bout, this is known as the repeated bout effect (RBE), a phenomenon that results in large non-specific translational and transcriptional responses. Resistance exercise training (RET) stimulates similar adaptations which becomes more refined with continuing training. It was hypothesized that RET and RBE may share a common mechanism involving remodeling of the extracellular matrix (ECM). Healthy young adults (6 female, 10 males; 20.9 4.1 years) performed 6 sessions of unilateral RET with the contralateral leg acting as a control prior to 2 bilateral bouts (B1, B2) of 300 eccentric knee extensions separated by 21 d. Bilateral muscle biopsies were obtained before and 48-hours after each bout. Muscle damage assessed as the area under the curve for isometric force loss over 72-hours following ECC, was attenuated in B1 by RET (42%±4.76, p=0.035). Protection conferred by B1, resulted in complete recovery of force loss by 48h. ECC elevated expression of Transforming Growth Factor-Beta (TGF𝛽, p=0.018) and Collagen Type IV (COL4A1, p=0.027) only in the RET leg after each bout, while Matrix Metalloproteinase-2 (MMP2) mRNA increased after ECC only in the control leg (p=0.049). These results collectively suggest that short-term RET modifies the transcriptional response of ECM regulators to bouts of high volume ECC exercise which may partially underpin the observed attenuation of ECC induced EIMD by prior RET. Further analysis is required to determine if altered transcription of ECM regulators is also reflected at the protein level.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/83167
note: SHORT-TERM RESISTANCE EXERCISE TRAINING ATTENUATES MUSCLE DAMAGE AND ALTERS TRANSCRIPTION OF MUSCLE EXTRACELLULAR MATRIX REGULATORS IN RESPONSE TO 300 ECCENTRIC CONTRACTIONS IN YOUNG ADULTS by CHING WAH DONNA LI B.A.S., University of Guelph, 2020 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Kinesiology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) November 2022 © Ching Wah Donna Li, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Short-term resistance exercise training attenuates muscle damage and alters transcription of muscle extracellular matrix regulators in response to 300 eccentric contraction in young adults submitted by Ching Wah Donna Li in partial fulfilment of the requirements for the degree of Master of Science in Kinesiology Examining Committee: Dr. Cameron Mitchell, Professor, Kinesiology, UBC Supervisor Dr., Don McKenzie, Professor, Kinesiology, UBC Supervisory Committee Member Dr., James Markworth, Professor, Animal Sciences, Purdue University Supervisory Committee Member iii Abstract A maximal eccentric (ECC) bout can confer protection by reducing exercise-induced muscle damage (EIMD) following a subsequent ECC bout, this is known as the repeated bout effect (RBE), a phenomenon that results in large non-specific translational and transcriptional responses. Resistance exercise training (RET) stimulates similar adaptations which becomes more refined with continuing training. It was hypothesized that RET and RBE may share a common mechanism involving remodeling of the extracellular matrix (ECM). Healthy young adults (6 female, 10 males; 20.9 4.1 years) performed 6 sessions of unilateral RET with the contralateral leg acting as a control prior to 2 bilateral bouts (B1, B2) of 300 eccentric knee extensions separated by 21 d. Bilate…
has type: Thesis/Dissertation
date issued: 2023-05
is shown at: 10.14288/1.0422010
language: eng
degree discipline: Kinesiology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Mitchell, Cameron J.
type: Text
identifier URI: http://hdl.handle.net/2429/83167