Characterization of two regulators in plant immune receptor-mediated defense
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Ao, Kevin
issued: 2022-10-27T16:22:23Z
issued: 2022
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Plants possess a complex multi-layered immune system that enables them to defend themselves against many potential invaders. However, some virulent pathogens can overcome these defenses to cause plant disease. Plant pathogens are a major obstacle to efficient and sustainable global food production. Understanding the underlying molecular mechanisms behind plant immunity will allow precise science-based engineering and breeding of plant disease resistance. Nucleotide-binding leucine-rich repeat proteins (NLRs) are one type of immune receptor that mediate the recognition of pathogens and the activation of defense responses. Although many regulators contributing to NLR homeostasis and activation have been uncovered, our understanding of plant immune signaling is far from complete. One limitation that has hindered gene discovery is genetic redundancy, which can mask immune phenotypes in single mutant plants. Using ectopic overexpression and CRISPR/Cas9-mediated targeted deletion strategies for reverse genetic screens, these limitations can be overcome. In this study, two novel regulators involved in NLR immunity were characterized. The first gene, called SNIPER7 (SNC1-INFLUENCING PLANT E3 LIGASE REVERSE GENETIC 7), was isolated from a previous E3 ubiquitin ligase overexpression screen. SNIPER7 overexpression results in constitutive activation of NLR-mediated defense. SNIPER7 targets the conserved unfoldase CDC48A (CELL DIVISION CYCLE 48A) for degradation. Since CDC48A is involved in the homeostasis of the NLR SNC1 (SUPPRESSOR OF NPR1-1, CONSTITUTIVE 1), SNIPER7 may contribute to immune receptor accumulation upon pathogen infection. The second gene, called TC1b (TRAF CANDIDATE 1b), was isolated from a new CRISPR/Cas9 screen targeting TRAF (TUMOR NECROSIS FACTOR RECEPTOR ASSOCIATED FACTOR) domain containing genes. TRAF domain genes are hypothesized to play a role in plant NLR immunity since many mammalian TRAF domain proteins are involved in innate immunity. TC1b is requir…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/83070
note: CHARACTERIZATION OF TWO REGULATORS IN PLANT IMMUNE RECEPTOR-MEDIATED DEFENSE by KEVIN AO B.Sc. (Honours), The University of British Columbia, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Botany) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) October 2022 © Kevin Ao, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Characterization of Two Regulators in Plant Immune Receptor-Mediated Defense submitted by Kevin Ao in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Botany Examining Committee: Dr. Xin Li, Professor, Botany, UBC Supervisor Dr. Marcel Wiermer, Professor, Biochemistry of Plant-Microbe Interactions, Georg-August-University Göttingen Supervisory Committee Member Dr. James Kronstad, Professor, Microbiology and Immunology, UBC University Examiner Dr. Susan Murch, Professor, Biochemistry and Molecular Biology, Chemistry, UBC-Okanagan University Examiner Dr. Ping He, Professor, Biochemistry & Biophysics, Texas A&M University External Examiner Additional Supervisory Committee Members: Dr. Ljerka Kunst, Professor, Botany, UBC Supervisory Committee Member Dr. Cara Haney, Associate Professor, Microbiology and Immunology, UBC Supervisory Committee Member iii Abstract Plants possess a complex multi-layered immune system that enables them to defend themselves against many potential invaders. However, some virulent pathogens can overcome these defenses to cause plant disease. Plant pathogens are a major obstacle to efficient and sustainable global food production. Understanding the underlying molecular mechanisms behind plant immunity will allow precise science-based engineering and breeding of plant disease resistance. Nucleotide-binding leucine-rich repeat proteins (NLRs) are one type of immune receptor …
has type: Thesis/Dissertation
date issued: 2023-05
is shown at: 10.14288/1.0421623
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Li, Xin
type: Text
identifier URI: http://hdl.handle.net/2429/83070