Exploring Cannabis sativa's response to powdery mildew (Golovinomyces ambrosiae) infection
department or school: Science, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Quesada-Ramirez, Lucia
issued: 2023-09-30T07:00:00Z
issued: 2022
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Powdery mildew infection, which is caused by the fungal pathogen Golovinomyces ambrosiae (Erysiphaceae), has been extensively characterized in Arabidopsis (Brassicaceae), and other species, but not in Cannabis sativa (Cannabaceae), a commercially important plant species. In this thesis, I explore the response of C. sativa to powdery mildew infection. I used microscopy to examine the phenotypic response and RNA-sequencing to examine the transcriptomic response to powdery mildew. I also examined differences between resistant (AGA10) and susceptible (Purple Kush) genotypes. Microscopy revealed that there are differences between the two genotypes in germination and hypersensitive response (programmed cell death). RNA-sequencing revealed differences in expression of genes encoding enzymes of the phenylpropanoid and monolignol biosynthetic pathways, phytoalexin production, and other plant-pathogen responses. Gene ontology (GO) functional analysis shows that the two genotypes also differ in expression of genes related to carbohydrate metabolic processing and other enzymatic processes. I conclude that a likely main factor behind the AGA10 resistant phenotype is increased reactive oxygen species and hypersensitive response, due to earlier or increased expression of genes such as oxidoreductases, peroxidases, phenylalanine ammonia lyase and trans-cinnamate 4-monooxygenase.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/82626
note: EXPLORING CANNABIS SATIVA’S RESPONSE TO POWDERY MILDEW (GOLOVINOMYCES AMBROSIAE) INFECTION by Lucia Quesada-Ramirez B.Sc., The University of British Columbia, 2019 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Genome Science and Technology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2022 © Lucia Quesada-Ramirez, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Exploring Cannabis sativa’s response to powdery mildew (Golovinomyces ambrosiae) infection submitted by Lucia Quesada-Ramirez in partial fulfilment of the requirements for the degree of Master of Science in Genome Science and Technology Examining Committee: Lacey Samuels, Professor, Botany, UBC Co-supervisor Loren Rieseberg, Professor, Botany, UBC Co-supervisor Richard Hamelin, Professor, Department Head, Forest and Conservation Sciences, UBC Supervisory Committee Member Sean Graham, Professor, Botany, UBC Additional Examiner Additional Supervisory Committee Members: Joerg Bohlmann, Professor, Forest and Conservation Sciences, UBC Supervisory Committee Member iii Abstract Powdery mildew infection, which is caused by the fungal pathogen Golovinomyces ambrosiae (Erysiphaceae), has been extensively characterized in Arabidopsis (Brassicaceae), and other species, but not in Cannabis sativa (Cannabaceae), a commercially important plant species. In this thesis, I explore the response of C. sativa to powdery mildew infection. I used microscopy to examine the phenotypic response and RNA-sequencing to examine the transcriptomic response to powdery mildew. I also examined differences between resistant (AGA10) and susceptible (Purple Kush) genotypes. Microscopy revealed that there are differences between the two genotypes in germination and hypersensitive response (programmed cell death). RNA-…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0418428
language: eng
degree discipline: Genome Science and Technology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Samuels, Anne Lacey, 1962-
contributor: Rieseberg, Loren H.
type: Text
identifier URI: http://hdl.handle.net/2429/82626