Role of phenolic acids on intestinal epithelial cell signaling and functionality
department or school: Land and Food Systems, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Mu, Kaiwen
issued: 2023-09-30T07:00:00Z
issued: 2022
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Phenolic compounds derived from the diet are typically sourced from cereals, fruits and vegetables and also beverages. They are documented to have biological functions that include antioxidant and anti-inflammatory activities, and can mitigate gastrointestinal disorders. This thesis focused on looking at mechanism(s) of phenolic acid induced intracellular hydrogen peroxide (H₂O₂) level and effects on intestinal epithelial cell signaling that are related to intestinal epithelial functionality. The first experiment showed that different phenolic acids varied specifically in relative stability and capacity to produce H₂O₂ when incubated in cell culture media for 24 hr. Phenolic acids varied in affinity to induce apoptosis and inhibit proliferation in non-differentiated Caco-2 cells. Subsequent studies demonstrated the effect of selective phenolic acids on extracellular/intracellular redox by controlling H₂O₂ influx activity and to relate this event to cell signaling and control of oxidative stress in differentiated Caco-2 cells. HyPer-3, a genetically encoded fluorescent H₂O₂ sensitive indicator was constructed to monitor intracellular H₂O₂ in differentiated Caco-2 cells. Results showed that extracellular generation of H₂O₂ occurred from autoxidation of phenolic acids in culture and subsequent disappearance corresponded to intracellular uptake in differentiated Caco-2 cells. Phenolic acid specific pro-oxidant activity was sufficient to activate intracellular nuclear factor-erythroid factor 2-related factor 2 (Nrf2) cell signaling; the degree of which was dependent on both the source of the phenolic acid and concentration used in culture. A Caco-2/RAW 264.7 co-culture model enabled intercellular cell signaling measurements in response to an inflammatory response by inflamed macrophage. The capacity of specific phenolic acids to regulate the inflammatory status of activated RAW 264.7 cells was dependent on the type of phenolic acid which influenced Nrf2 cell signaling, m…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/82583
note: ROLE OF PHENOLIC ACIDS ON INTESTINAL EPITHELIAL CELL SIGNALING AND FUNCTIONALITY by KAIWEN MU BSc, University of Manitoba, 2015 MFS, The University of British Columbia, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF Doctor of Philosophy in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Food Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2022 © Kaiwen Mu, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Role of phenolic acids on intestinal epithelial cell signaling and functionality submitted by Kaiwen Mu in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Food Science Examining Committee: Dr. David D Kitts, Food Science, UBC Supervisor Dr. Siyun Wang, Food Science, UBC Supervisory Committee Member Dr. Simone Castellarin, Plant Science, UBC University Examiner Dr. Barbara, Stefanska, Human Nutrition, UBC University Examiner Additional Supervisory Committee Members: Dr. Rickey Yada, Food Science, UBC Supervisory Committee Member Dr. Zhaoming Xu, Human Nutrition, UBC Supervisory Committee Member iii Abstract  Phenolic compounds derived from the diet are typically sourced from cereals, fruits and vegetables and also beverages. They are documented to have biological functions that include antioxidant and anti-inflammatory activities, and can mitigate gastrointestinal disorders. This thesis focused on looking at mechanism(s) of phenolic acid induced intracellular hydrogen peroxide (H2O2) level and effects on intestinal epithelial cell signaling that are related to intestinal epithelial functionality. The first experiment showed that different phenolic acids varied specifically in relative stability and capacity to produce H2O2 when incubated in cell culture media for 24 hr. Phenolic acids varied in affinity to induce apoptosis and inhibit proliferation in non-dif…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0417592
language: eng
degree discipline: Food Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Kitts, David D.
type: Text
identifier URI: http://hdl.handle.net/2429/82583