The evaluation of cold-stored platelets
department or school: Medicine, Faculty of
department or school: Pathology and Laboratory Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Zhao, Han Qi
issued: 2022-08-19T20:18:07Z
issued: 2022
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Platelets are an integral part of transfusion medicine. In Canada, over 80% of the platelet units for transfusion are produced via the buffy coat method from whole blood and stored in plasma. The storage condition is at 22°C under constant agitation for a maximum of 7 days. These are known as room temperature stored platelets (RPs). There are several disadvantages associated with RPs. First, RPs experience a significant decrease in platelet quality during storage known as the platelet storage lesion. Second, RPs have increased risks of microorganism growth. Last, RPs have a very limited shelf life which contributes to product wastage. Platelets stored at 4°C or cold-stored platelets (CPs) have been gaining renewed interest due to their potential of overcoming the disadvantages of RPs. Preliminary studies have shown that CPs have preserved mitochondrial functions, superior hemostatic functions and stable pH compared to RPs. Additionally, CPs have been hypothesized to be a superior product especially for bleeding patients. In this thesis, we compared the in vitro storage characteristics of CPs versus RPs over 14 days of storage. We showed that CPs have better aggregation response to physiological agonists and formed stronger clots compared to RPs. We also examined the underlying biological changes of CPs and RPs during storage by performing an untargeted metabolomic study. We showed that CPs exhibited significantly less metabolomic stress during storage compared to RPs as exemplified by the stable reduced glutathione pools in the CPs but not RPs. Additionally, we explored the potential benefits of CPs in bleeding scenarios by using an in vitro trauma transfusion model. From this experiment, we showed that donor whole blood mixed with CPs is more resistant to hyperfibrinolysis that is commonly observed in trauma. Finally, we explored the importance of oxidative stress in platelet storage by inhibiting the activity of the glucose 6-phosphate dehydrogenase (G6PD) in the …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/82424
note: THE EVALUATION OF COLD-STORED PLATELETS by Han Qi Zhao BSc (Hons), University of Toronto, 2014 MSc, University of British Columbia, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in The Faculty of Graduate and Postdoctoral Studies (Pathology and Laboratory Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2022 ©Han Qi Zhao, 2022ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: The evaluation of cold-stored platelets submitted by Han Qi Zhao in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Pathology and Laboratory Medicine Examining Committee: Dr. Dana Devine, Professor, Pathology and Laboratory Medicine, UBC Supervisor Dr. Suzanne Vercauteren, Clinical Professor, Pathology and Laboratory Medicine, UBC Supervisory Committee Member Dr. Hugh Kim, Associate Professor, Biochemistry and Molecular Biology, UBC University Examiner Dr. Cedric Carter, Associate Professor Emeritus, Pathology and Laboratory Medicine, UBC University Examiner Additional Supervisory Committee Members: Dr. Ed Conway, Professor, Pathology and Laboratory Medicine, UBC Supervisory Committee Member Dr. Christian Kastrup, Associate Professor, Biochemistry and Molecular Biology, UBC Supervisory Committee Member iii Abstract Platelets are an integral part of transfusion medicine. In Canada, over 80% of the platelet units for transfusion are produced via the buffy coat method from whole blood and stored in plasma. The storage condition is at 22°C under constant agitation for a maximum of 7 days. These are known as room temperature stored platelets (RPs). There are several disadvantages associated with RPs. First, RPs experience a significant decrease in platelet quality during storage known as the platelet storage lesion. Second, RPs have increased risks of microorganism growth. Last,…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0417448
language: eng
degree discipline: Pathology and Laboratory Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Devine, Dana V.
type: Text
identifier URI: http://hdl.handle.net/2429/82424