Engineering dynamic protein-based hydrogels
department or school: Science, Faculty of
department or school: Chemistry, Department of
data provider: DSpace
degree campus: UBCV
creator: Duan, Tianyu
issued: 2022-08-16T23:17:09Z
issued: 2022
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Hydrogels are water-containing soft materials made of cross-linked polymer networks. Hydrogels can emulate many features of native tissues and organisms, and have therefore found many applications in biomedical studies. Recently, driven by the needs for stimuli-responsive materials in various biomedical applications, “dynamic” hydrogels that can change their physical, chemical, biological, or mechanical properties have attracted increasing interest. Recombinant proteins are promising hydrogel building blocks due to their biocompatibility, diverse biofunctions, and designable structures. Moreover, stimuli-responsive conformational changes and interactions of proteins provide possibilities for constructing dynamic protein-based hydrogels. This thesis reviews the state-of-the-art studies of dynamic protein-based hydrogels, including the cross-linking methods, the design principles, and the future directions of this field. Driven by the needs for more predictable, sensitive, and reversible hydrogels, we engineer novel protein-based hydrogels in this thesis. An elastomeric protein-based hydrogel is first engineered with thermo-responsive properties, which is controlled by the thermo-responsive in situ phase transition of the polypeptide side chains in the hydrogel network. Then, two dynamic hydrogels are physically cross-linked by a coiled coil interaction and a protein-protein interaction, respectively, which both exhibit time-dependent viscoelastic properties, multiple energy dissipation modes, and injectability. The viscoelastic properties of these two physically cross-linked hydrogels are stimuli-responsive due to the stimuli-responsive nature of their cross-linking methods. Finally, a light-controlled information writing approach is developed by using a light-responsive protein-protein interaction to decorate fluorescent proteins onto protein-based hydrogel blank slates. Consequently, 2D/3D fluorescence images can be produced and stored in protein-based hydrogels. T…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/82372
note: ENGINEERING DYNAMIC PROTEIN-BASED HYDROGELS by Tianyu Duan B.Sc., Beijing Normal University, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Chemistry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2022 © Tianyu Duan, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Engineering Dynamic Protein-Based Hydrogels submitted by Tianyu Duan in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry Examining Committee: Hongbin Li, Professor, Chemistry, UBC Supervisor Jayachandran Kizhakkedathu, Professor, Pathology, Chemistry, UBC Supervisory Committee Member Carl Michal, Associate Professor, Physics, Chemistry, UBC University Examiner Mark Thachuk, Associate Professor, Chemistry, UBC University Examiner Additional Supervisory Committee Members: Katherine Ryan, Associate Professor, Chemistry, UBC Supervisory Committee Member Suzana Straus, Professor, Chemistry, UBC Supervisory Committee Member iii Abstract Hydrogels are water-containing soft materials made of cross-linked polymer networks. Hydrogels can emulate many features of native tissues and organisms, and have therefore found many applications in biomedical studies. Recently, driven by the needs for stimuli-responsive materials in various biomedical applications, “dynamic” hydrogels that can change their physical, chemical, biological, or mechanical properties have attracted increasing interest. Recombinant proteins are promising hydrogel building blocks due to their biocompatibility, diverse biofunctions, and designable structures. Moreover, stimuli-responsive conformational changes and interactions of proteins provide possibilities for constructing dynamic protein-based hydrogels. This thesis reviews the state-of-the-art studies of dynamic pro…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0417329
language: eng
degree discipline: Chemistry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Li, Hongbin
type: Text
identifier URI: http://hdl.handle.net/2429/82372