Analysis of the Arabidopsis synaptotagmin gene expression and protein accumulation during stress using luciferase-based markers
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Strelau, Matthew
issued: 2022-06-02T17:11:18Z
issued: 2022
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The responses of plants to adverse environmental conditions require the adequate sensing of stress signals from the environment and their integration into coordinated cellular responses. These coordinated responses in plants often involve information exchanges between intracellular organelles that rely on vesicular trafficking and/or non-vesicular transport at membrane contact sites. This thesis focuses on the plant endoplasmic reticulum (ER) plasma membrane (PM) contact sites (EPCS), which are evolutionarily conserved nanostructures that facilitate exchanges of information between the cortical ER and the PM. The close association between membranes at EPCS is facilitated by physical tethering often mediated by lipid-transfer proteins. Members of one of these families, known as the plant synaptotagmins, are important elements conferring tolerance to biotic and abiotic stresses in Arabidopsis. Still, little is known about the regulatory mechanisms and environmental triggers controlling the expression and activity of the different synaptotagmins in plants. In this thesis, I use luciferase-based markers to perform systematic analyses of the SYNAPTOTAGMIN1 (SYT1) promoter activity and protein accumulation in Arabidopsis. My results show that SYT1 expression increases in response to salt-induced ionic and osmotic stresses, particularly when the stress generator contains Cl-. Remarkably, the amount of SYT1 protein does not change in response to salt stress indicating that SYT1 transcriptional and translational levels are uncoupled. By measuring the stability of SYT1 in response to NaCl, I determined that salt stress has an increased effect on the stability of the SYT1 protein and that its degradation is partially controlled by the 26S ubiquitin proteasome. I conducted an in silico analyses of gene expression for different members of the Arabidopsis synaptotagmin family and validated the results using SYT promoter - GUS fusions. My results show that while some Arabidopsis S…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/81684
note: ANALYSIS OF THE ARABIDOPSIS SYNAPTOTAGMINS GENE EXPRESSION AND PROTEIN ACCUMULATION DURING STRESS USING LUCIFERASE-BASED MARKERS by MATTHEW STRELAU B.Sc., Trinity Western University, 2017 A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (BOTANY) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) May 2022 © Matthew Strelau, 2022 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: ANALYSIS OF THE ARABIDOPSIS SYNAPTOTAGMINS GENE EXPRESSION AND PROTEIN ACCUMULATION DURING STRESS USING LUCIFERASE-BASED MARKERS submitted by MATTHEW STRELAU in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in BOTANY Examining Committee: Abel Rosado, Associate Professor, Botany, UBC Supervisor George Haughn, Professor, Botany, UBC Supervisory Committee Member Lacey Samuels, Professor, Head of Botany, UBC Supervisory Committee Member Simone Castellarin, Associate Professor, Land and Food Systems, UBC Additional Examiner Additional Supervisory Committee Members: Geoffrey Wasteneys, Professor, Botany, UBC Supervisory Committee Member iii Abstract The responses of plants to adverse environmental conditions require the adequate sensing of stress signals from the environment and their integration into coordinated cellular responses. These coordinated responses in plants often involve information exchanges between intracellular organelles that rely on vesicular trafficking and/or non-vesicular transport at membrane contact sites. This thesis focuses on the plant endoplasmic reticulum (ER) plasma membrane (PM) contact sites (EPCS), which are evolutionarily conserved nanostructures that facilitate exchanges of information between the cortical ER and the PM. The close association between membranes at EPCS is facilitated by physical tethering often mediated by lipi…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0413757
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Rosado, Abel
type: Text
identifier URI: http://hdl.handle.net/2429/81684