Non-canonical enzymes involved in cuticular wax biosynthesis : weaving the coat of Arabidopsis and cereal shoots
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Sun, Yulin
issued: 2021-09-24T21:30:08Z
issued: 2021
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Plant cuticles play important roles in plant development, fertility, and adaptation. The cuticular waxes directly facing the environment are made mainly from derivatives of very-long-chain (VLC) aliphatics. Previous studies on cuticular waxes clarified the biosynthesis of ubiquitous wax components based on the model plant Arabidopsis thaliana. However, the biosynthesis and chemical structures of numerous uncommon wax components from different plant species are still unclear. In my Ph.D. studies, I focused on wax components from both Arabidopsis thaliana and Poaceae crop species to identify new enzymes involved in plant cuticular wax biosynthesis and further characterize their biochemical functions. In chapter 2, I investigated alkane biosynthesis in bread wheat. The linkage of alkane content and transcriptome data revealed a candidate gene, TaCER1-1A. The phenotype of corresponding nullisomic-tetrasomic substitution wheat lines and heterologous expression of TaCER1-1A in rice and Arabidopsis confirmed its function in alkane synthesis. In chapter 3, the products associated with β-diketones in barley were profiled. New diketone homologs, the 2-alkanol ester profile and the ¹³C isotope abundance of different wax components indicated the unique biosynthesis pathway to β-diketones. The biochemical functions of the core enzymes on this pathway, DIKETONE METABOLISM HYDROLASE (DMH) and DIKETONE METABOLISM POLYKETIDE SYNTHASE (DMP), were investigated. In vivo and in vitro assays showed the direct condensation between 3-ketoacid and fatty acyl-CoA catalyzed by DMP, thus revealing the unique character of DMP as a PKS family member and revised the model of the β-diketone synthesis pathway in barley. In chapter 4, VLC alkenes from Arabidopsis young leaves were analyzed. Different from other plant species, alkenes ranging from C₃₃ to C₃₉ with predominance of 7- and 9- isomers were found in Arabidopsis. An acyl-CoA desaturase family member, ADS4.2, was shown to influence alkene fo…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/79795
note: NON-CANONICAL ENZYMES INVOLVED IN CUTICULAR WAX BIOSYNTHESIS: WEAVING THE COAT OF ARABIDOPSIS AND CEREAL SHOOTS by Yulin Sun B.Sc., Northwest A&F University, 2012M.Sc., Northwest A&F University, 2015A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (BOTANY) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) September 2021© Yulin Sun, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Non-canonical enzymes involved in cuticular wax biosynthesis: weaving the coat of Arabidopsis and cereal shoots submitted by Yulin Sun in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Botany Examining Committee: Dr. Reinhard Jetter, Botany, Chemistry, UBC Supervisor Dr. Lacey Samuels, Botany, UBC Supervisory Committee Member Dr. Simone Castellarin, Land and Food Systems, UBC University Examiner Dr. Katherine Ryan, Chemistry, UBC University Examiner Additional Supervisory Committee Members: Dr. Abel Rosado, Botany, UBC Supervisory Committee Member Dr. Jörg Bohlmann, Botany, UBC Supervisory Committee Member iii Abstract Plant cuticles play important roles in plant development, fertility, and adaptation. The cuticular waxes directly facing the environment are made mainly from derivatives of very-long-chain (VLC) aliphatics. Previous studies on cuticular waxes clarified the biosynthesis of ubiquitous wax components based on the model plant Arabidopsis thaliana. However, the biosynthesis and chemical structures of numerous uncommon wax components from different plant species are still unclear. In my Ph.D. studies, I focused on wax components from both Arabidopsis thaliana and Poaceae crop species to identify new enzymes involved in plant cuticular wax biosynthesis and further characterize their biochemical functions. In chapter 2, I investi…
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0402340
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Jetter, Reinhard, 1962-
type: Text
identifier URI: http://hdl.handle.net/2429/79795