Dental management of herpes simplex virus (HSV-1 & HSV-2) using n-butyl and 2-octyl cyanoacrylate as a barrier : a pilot study
department or school: Dentistry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Lan, Yolanda Haruka
issued: 2021-09-24T15:37:02Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Objectives: Herpes simplex virus (HSV-1, 2) can present as symptomatic oral lesions or asymptomatic shedding. The risk of viral spreading during dental procedures is a safety concern, which we hypothesized may be reduced using cyanoacrylate adhesive (CA). The study aimed to determine 1) the magnitude of asymptomatic reactivation compared to symptomatic and 2) the efficacy of CA in limiting the virus transmission in symptomatic oral lesions. Methods: Thirty-three participants were recruited from UBC-Faculty of Dentistry. Oral mucosal (OS) and lesion swabs (LS) were collected and tested for HSV-1 and 2 using polymerase chain reaction (PCR) assay. Asymptomatic participants with possible history of oral HSV lesions (n=17) were followed for three months. Symptomatic lesions (n=11) had OS and LS collected, CA applied and subjected to soft tissue manipulation (STM). CA’s efficacy to alleviate/cause pain was assessed by daily journaling using visual analogue scale (VAS: 0-10). Examiner was blinded to symptomatic LS results and statistical analyses were performed using SPSS 27. Results: Twenty-one participants completed the study. One case of asymptomatic reactivation was detected out of 17 asymptomatic participants (2.0% reactivation rate). Data on 11 lesions were collected. Correlations between various lesion clinical parameters were assessed. Both lesion size and lesion PCR cycle threshold value (Ct) (p=0.04) and lesion day and stage (p<0.001) were positively correlated. Lesion discomfort and lesion stage were negatively correlated (p<0.03). Mean of reported average discomfort was 1.16±1.11, out of 10. Significant differences were detected in Ct of OS and LS (p<0.02), OS and post-STM swab (p<0.03). LS was observed to have lower Ct than post-PA swab. PA was accepted by participants with mean inverse-VAS score of 1.61±1.52. PA remained intact for an average of 25.25±17.63 hours. No adverse reactions to PA were reported. Side-effects include feeling of localized tightness an…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/79792
note: DENTAL MANAGEMENT OF HERPES SIMPLEX VIRUS (HSV-1 & HSV-2) USING N-BUTYL AND 2-OCTYL CYANOACRYLATE AS A BARRIER: A PILOT STUDY by Yolanda Haruka Lan BDSc., The University of British Columbia, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Craniofacial Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) September 2021 © Yolanda Haruka Lan, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis entitled: Dental management of herpes simplex virus (HSV-1 & HSV-2) using n-butyl & 2-octyl cyanoacrylate as a barrier: a pilot study submitted by Yolanda Haruka Lan in partial fulfillment of the requirements for the degree of Master of Science in Craniofacial Science Examining Committee: Penny Hatzimanolakis, Clinical Associate Professor, Oral Biological & Medical Sciences, UBC Supervisor Dr. Lari Häkkinen, Professor, Oral Biological & Medical Sciences, UBC Supervisory Committee Member Dr. Denise Laronde, Associate Professor, Oral Biological & Medical Sciences, UBC Supervisory Committee Member Dr. Eli Whitney, Associate Professor of Teaching, Oral Biological & Medical Sciences, UBC Supervisory Committee Member Dr. Vincent Lee, Assisting Professor of Teaching, Oral Health Sciences, UBC Additional Examiner iii Abstract Objectives: Herpes simplex virus (HSV-1, 2) can present as symptomatic oral lesions or asymptomatic shedding. The risk of viral spreading during dental procedures is a safety concern, which we hypothesized may be reduced using cyanoacrylate adhesive (CA). The study aimed to determine 1) the magnitude of asymptomatic reactivation compared to symptomatic and 2) the efficacy of CA in limiting the virus transmission in symptomatic oral lesions. Methods: Thirty-three participants were recruited from UBC-Faculty of Dentistry. Oral mucosal (OS) and lesion swabs (L…
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0402337
language: eng
degree discipline: Craniofacial Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Hatzimanolakis, Penny
type: Text
identifier URI: http://hdl.handle.net/2429/79792