The influence of different plasma lipoprotein fractions and macrophages on smooth muscle cell foam cell formation
department or school: Medicine, Faculty of
department or school: Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Ortega, Carleena Angelica S.
issued: 2021-08-26T21:28:54Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Atherosclerosis, which manifests clinically as ischemic cardiovascular disease (CVD), is the leading cause of global mortality. From a lipid standpoint, CVD risk has traditionally been assessed by measuring low-density lipoprotein (LDL) cholesterol in the fasted state. Recently, both the measurement of the complete non-high-density lipoprotein (non-HDL) cholesterol fraction and the lipids in the nonfasting state have gained traction as better indicators of CVD risk and prevention. In addition, while macrophages in atherosclerotic lesions have been the focus of most plaque cholesterol metabolism studies, growing evidence suggests that smooth muscle cells (SMCs) are the key cell type driving initial lesion development and the main cell type contributing to cholesterol-overloaded “foam cells” in these lesions. Further evidence suggests that macrophages and SMCs may influence each other’s tendency to become foam cells. The current study’s aims are to compare the effects of LDL and non-HDL from both fasting and nonfasting plasma on the lipid loading of SMCs and macrophages, and the effects of SMC-macrophage coculture on the tendency of each cell type to become foam cells in vitro. We hypothesized that the nonfasting lipoproteins, the non-HDL fractions, and coculture with macrophages will promote more SMC foam cells compared with the fasting lipoproteins, LDL fractions, and monoculture. Our results show that lipoprotein loading of human SMCs have a trend of higher cholesterol accumulation with fasting and non-HDL fractions via cholesterol mass quantification with mass spectrometry. However, flow cytometry analysis demonstrated a trend of more BODIPY-high SMCs with the nonfasting and non-HDL fractions whether in monoculture or direct co-culture with macrophages. Coculture demonstrated a trend of more BODIPY-high SMCs in the presence of the non-fasting non-HDL compared with the monocultured SMCs. Monocultured macrophage demonstrated a trend of higher cholesterol accumulatio…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/79469
note: THE INFLUENCE OF DIFFERENT PLASMA LIPOPROTEIN FRACTIONS AND MACROPHAGES ON SMOOTH MUSCLE CELL FOAM CELL FORMATION by Carleena Angelica S. Ortega B.S., The University of Waterloo, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2021 © Carleena Angelica S. Ortega, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: The Influence of Different Plasma Lipoprotein Fractions and Macrophages on Smooth Muscle Cell Foam Cell Formation submitted by Carleena Angelica S. Ortega in partial fulfillment of the requirements for the degree of Master of Science in Experimental Medicine Examining Committee: Gordon Francis, Department of Medicine Supervisor Pascal Bernatchez, Department of Anesthesiology, Pharmacology and Therapeutics Supervisory Committee Member Edward Pryzdial, Department of Pathology and Laboratory Medicine Additional Examiner Additional Supervisory Committee Members: John Boyd, Department of Medicine Supervisory Committee Member Mari DeMarco, Department of Pathology and Laboratory Medicine Supervisory Committee Member iii Abstract Atherosclerosis, which manifests clinically as ischemic cardiovascular disease (CVD), is the leading cause of global mortality. From a lipid standpoint, CVD risk has traditionally been assessed by measuring low-density lipoprotein (LDL) cholesterol in the fasted state. Recently, both the measurement of the complete non-high-density lipoprotein (non-HDL) cholesterol fraction and the lipids in the nonfasting state have gained traction as better indicators of CVD risk and prevention. In addition, while macrophages in atherosclerotic lesions have been the focus of most plaque cholesterol metabolism studies, growing evidence suggests that smooth muscl…
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0401732
language: eng
degree discipline: Experimental Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Francis, Gordon A.
type: Text
identifier URI: http://hdl.handle.net/2429/79469