Light-based bioprinting for fabricating vascularized tissues
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Kumar, Hitendra
issued: 2021-07-19T21:51:11Z
issued: 2021
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: The natural tissues are architecturally complex and heterogenous in nature and comprise of a dense vasculature for facilitating nutrient and chemokines transport. To mimic the functionality of natural tissues, bioprinting methods should be able to capture the tissue heterogeneity and create a vascularized scaffold. Stereolithography (SLA) bioprinting is a promising process to fabricate high-resolution anatomically accurate tissue analogues. However, non-optimized bioinks and poor design of the scaffolds limit the capability of SLA bioprinting. In view of these challenges, a method to obtain SLA bioprinting compatible gelatin methacryloyl (GelMA) bioinks which maintain a low-viscosity liquid state at room temperature is presented. The method considers GelMA synthesis parameters – solvent, pH and duration; to tune the end product. The obtained bioinks exhibit superior photopolymerization kinetics, biocompatibility and printability. Addition of a three-component photoinitiator further improved the sensitivity of the bioinks to visible light and resulted in an accelerated photopolymerization. The printing time was nearly halved and structurally stable structures could be formed with lower GelMA concentration. Next, a numerical model is developed to predict the hydrogel formation in mask photopolymerization. Considering a realistic Gaussian illumination and diffusive transport of chemical species within the bioink, the model was able to predict the printing artifacts like overcrosslinking and distortion in the hydrogel shape. In the last step, a vascular network generation method inspired from leaves and tree growth is presented. Biologically relevant vasculature was formed guided by medical image derived tissue structure and porosity. By studying nutrient transport, optimized networks with increased complexity were obtained. In combination, the described bioinks and the numerical models provide effective tools for designing the tissue mimics with embedded heterogeneity …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/79041
note: LIGHT-BASED BIOPRINTING FOR FABRICATING VASCULARIZED TISSUES by Hitendra Kumar M. Tech., Indian Institute of Technology Kanpur, 2016 B. Tech., Indian Institute of Technology Kanpur, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Mechanical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) June 2021 © Hitendra Kumar, 2021 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, the dissertation entitled: Light-based bioprinting for fabricating vascularized tissues submitted by Hitendra Kumar in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Mechanical Engineering. Examining Committee: Dr. Keekyoung Kim Department of Mechanical and Manufacturing Engineering University of Calgary Supervisor Dr. Kevin Golovin School of Engineering The University of British Columbia Co-supervisor Dr. Ian Foulds School of Engineering The University of British Columbia Supervisory Committee Member Dr. Frederic Menard Department of Chemistry The University of British Columbia Supervisory Committee Member Dr. Solomon Tesfamariam School of Engineering The University of British Columbia University Examiner Dr. Xiongbiao (Daniel) Chen Department of Mechanical Engineering University of Saskatchewan External Examiner iii Abstract The natural tissues are architecturally complex and heterogenous in nature and comprise of a dense vasculature for facilitating nutrient and chemokines transport. To mimic the functionality of natural tissues, bioprinting methods should be able to capture the tissue heterogeneity and create a vascularized scaffold. Stereolithography (SLA) bioprinting is a promising process to fabricate high-resolution anatomically accurate tissue analogues. However, non-optimized bioinks and poor design of the scaffolds limit the capability of SLA bioprinting. In view of these challenge…
has type: Thesis/Dissertation
date issued: 2021-09
is shown at: 10.14288/1.0400478
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Kim, Keekyoung
contributor: Golovin, Kevin
type: Text
identifier URI: http://hdl.handle.net/2429/79041