Gene duplication and expression of specialized metabolic pathway genes in Cannabis sativa
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Cizek, Christian
issued: 2022-06-30T07:00:00Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Cannabis sativa is now widely cultivated for recreational and medicinal markets (drug-type cannabis) in addition to fiber and grain for materials and foodstuffs (fiber-type cannabis). Cannabis often is grown for its female flowers, which are highly concentrated with the specialized metabolites cannabinoids and terpenes. These compounds are specifically found in glandular trichomes, which are abundant on the surfaces of maturing cannabis flowers. However, both the abundance and identity of specialized metabolites can vary considerably across different cannabis varieties. Drug-type cannabis commonly contains the cannabinoid, THC, and fiber-type cannabis contains CBD. In this research, 24 gene families from the biochemical pathways responsible for cannabinoid and terpenoid production were analyzed across five different genomes of Cannabis sativa, containing a diversity of chemical and physical phenotypes. Orthologous genes from hops and three other Rosales species also were included. Gene duplication patterns and copy number variation were investigated using phylogenetic trees to define the evolutionary patterns within these biochemical pathways. Additionally, the duplicated genes within these pathways were analyzed for gene expression in several organ types of the Purple Kush variety. When comparing the terpenoid and cannabinoid pathways, both the non-mevalonate (MEP) and mevalonate (MVA) pathways contained fewer duplicated genes than the genes involved in cannabinoid biosynthesis. The evolutionary origins of the olivetolic acid cyclase (OAC) and aromatic prenyltransferase (APT) genes were revealed when comparing with hops and other closely related species. The gene expression analysis of the Purple Kush cultivar indicated that genes involved in both terpenoid and cannabinoid pathways were expressed highest in flowers. However, the number of expressed copies and expression levels varied among genes, and different copies are expressed in different organ types. Overall,…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/78846
note: GENE DUPLICATION AND EXPRESSION OF SPECIALIZED METABOLIC PATHWAY GENES IN CANNABIS SATIVA by Christian Cizek B.Sc., Northwestern University, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Botany) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) June 2021 © Christian Cizek, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Gene Duplication and Expression of Specialized Metabolic Pathway Genes in Cannabis Sativa submitted by Christian Cizek in partial fulfillment of the requirements for the degree of Master of Science in Botany Examining Committee: Keith Adams, Professor, Botany, UBC Supervisor Joerg Bohlmann, Professor, Botany, UBC Supervisory Committee Member Quentin Cronk, Professor, Botany, UBC Supervisory Committee Member Simone Castellarin, Associate Professor, Land and Food Systems, UBC Additional Examiner iii Abstract Cannabis sativa is now widely cultivated for recreational and medicinal markets (drug-type cannabis) in addition to fiber and grain for materials and foodstuffs (fiber-type cannabis). Cannabis often is grown for its female flowers, which are highly concentrated with the specialized metabolites cannabinoids and terpenes. These compounds are specifically found in glandular trichomes, which are abundant on the surfaces of maturing cannabis flowers. However, both the abundance and identity of specialized metabolites can vary considerably across different cannabis varieties. Drug-type cannabis commonly contains the cannabinoid, THC, and fiber-type cannabis contains CBD. In this research, 24 gene families from the biochemical pathways responsible for cannabinoid and terpenoid production were analyzed across five different genomes of Cannabis sativa, containing a diversity of chemical and physical phenotypes. Orthologous genes from ho…
has type: Thesis/Dissertation
date issued: 2022-11
is shown at: 10.14288/1.0398736
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Adams, Keith L.
type: Text
identifier URI: http://hdl.handle.net/2429/78846