Exploring the role of Syndecan in Drosophila neural and glial development
department or school: Medicine, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Cheng, Duo
issued: 2021-06-03T21:33:33Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Glia are dynamic modulators required for the development and function of the nervous system, and various cell surface receptors which interpret the extracellular landscape are integral to their actions. However, the mechanisms by which glia accomplish such tasks remain to be fully characterized. This thesis explores the function of a major transmembrane heparan sulfate proteoglycan (HSPG) protein, Syndecan (Sdc), and its function in the Drosophila nervous system. In the central nervous system, where Syndecan is robustly expressed, I have shown that its downregulation in glia leads to a reduction in neuroblasts population, resulting in shrinkage of the brain lobe. In parallel, I observed elongation of the ventral nerve cord, indicative of disruption to the glial-ECM interactions. Syndecan is also found in high levels in various glial membranes within the peripheral nervous system, where I observed distinctive and cell-autonomous defects upon downregulation of Syndecan expression within each glial layer. Our results suggest Syndecan is required for the maintenance of blood-nerve barrier integrity, as Syndecan knockdown disrupted separate junction morphology of peripheral subperineurial glia. Moreover, I showed Syndecan is required for the ensheathment by multiple glial populations, including perineurial and wrapping glia. In particular, laminin deposition in the basal lamina surrounding the peripheral nerve was reduced upon knockdown of Syndecan in the outermost (perineurial) glial layer. Overall, these results demonstrate several novel aspects of Syndecan’s role in vivo during neural and glial development.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/78525
note: EXPLORING THE ROLE OF SYNDECAN IN DROSOPHILA NERUAL AND GLIAL DEVELOPMENT by DUO CHENG B.Sc., The University of Guelph, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (CELL AND DEVELOPMENTAL BIOLOGY) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) May 2021 © Duo Cheng, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis entitled: Exploring the role of Syndecan in Drosophila neural and glial development submitted by Duo Cheng in partial fulfillment of the requirements for the degree of Master of Science in Cell and developmental biology Examining Committee: Vanessa Auld, Professor, Zoology, UBC Supervisor Calvin Roskelley, Professor, Cellular and Physiological Sciences, UBC Supervisory Committee Member Matthew Ramer, Associate Professor, Zoology, UBC Supervisory Committee Member Hakima Moukhles, Associate Professor, Cellular and Physiological Sciences, UBC Additional Examiner iii Abstract Glia are dynamic modulators required for the development and function of the nervous system, and various cell surface receptors which interpret the extracellular landscape are integral to their actions. However, the mechanisms by which glia accomplish such tasks remain to be fully characterized. This thesis explores the function of a major transmembrane heparan sulfate proteoglycan (HSPG) protein, Syndecan (Sdc), and its function in the Drosophila nervous system. In the central nervous system, where Syndecan is robustly expressed, I have shown that its downregulation in glia leads to a reduction in neuroblasts population, resulting in shrinkage of the brain lobe. In parallel, I observed elongation of the ventral nerve cord, indicative of disruption to the glial-ECM interactions. Syndecan is also found in high levels in various glial membranes within the peripheral nervous syste…
has type: Thesis/Dissertation
date issued: 2021-11
is shown at: 10.14288/1.0398244
language: eng
degree discipline: Cell and Developmental Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Auld, Vanessa
type: Text
identifier URI: http://hdl.handle.net/2429/78525