The role of tubulobulbar complex-endoplasmic reticulum contact sites during junction turnover in the seminiferous epithelium
department or school: Medicine, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Adams, Arlo Cordell
issued: 2021-04-21T22:22:44Z
issued: 2021
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: The focus of this thesis is the characterization of the functions of the membrane contact sites (MCSs) between tubulobulbar complexes (TBCs) and the endoplasmic reticulum (ER). TBCs are double membrane endocytic invaginations that are endemic to Sertoli cells of the mammalian testis. They have been proposed to internalize intact intercellular junctions between late spermatids and Sertoli cells, allowing late spermatids to be released into the seminiferous tubule lumen. However, there is little data showing how TBCs may be regulated temporally and spatially to initiate junction turnover and subsequent spermatid release (spermiation). One of the most striking features of TBCs is the close association of ER to the ‘bulb’ portion, which is present for much of the time a TBC is developing and up until the moment of its vesiculation, all while it is still continuous with the plasma membrane. Although ER contacts are known to help regulate the form and function of other organelles such as endosomes, there have never been an investigation into what the functions of the TBC-ER contact sites are. It is my overall hypothesis that the TBC-ER contact sites are necessary for the normal development of TBCs into mature endocytic structures. Some of the well-established functions of ER contacts are both calcium and lipid exchange. Both processes have been shown to be fundamental to the progression of endosomes through the endosomal-lysosomal system. In this thesis, I begin by using qualitative approaches to establish that proteins known to be involved in both calcium and lipid exchange are present at TBC-ER contacts. I follow-up on my qualitative works with functional studies where I explore the role some of these proteins play in TBC development and in spermiation. Using in vivo knockdown experiments in rat testes to target two of these proteins (IP3R1 and ORP9), I demonstrate that both are necessary for the normal maturation of TBC bulbs and for sperm release to occur. These data …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/77889
note: THE ROLE OF TUBULOBULBAR COMPLEX-ENDOPLASMIC RETICULUM CONTACT SITES DURING JUNCTION TURNOVER IN THE SEMINIFEROUS EPITHELIUM by Arlo Cordell Adams B.Sc. (Hons), The University of British Columbia, 2015 A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in The Faculty of Graduate and Postdoctoral Studies (Cell and Developmental Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April, 2021 © Arlo Cordell Adams, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: The role of tubulobulbar complex-endoplasmic reticulum contact sites during junction turnover in the seminiferous epithelium submitted by Arlo Cordell Adams in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Cell and Developmental Biology Examining Committee: Dr. A Wayne Vogl, Cell and Developmental Biology, UBC Supervisor Dr. A Lacey Samuels, Botany, UBC Supervisory Committee Member Dr. Joy Richman, Craniofacial Science, UBC University Examiner Dr. Jeffrey Richards, Zoology, UBC University Examiner Additional Supervisory Committee Members: Dr. Edwin Moore, Cell and Developmental Biology, UBC Supervisory Committee Member Dr. Christopher Loewen, Cell and Developmental Biology, UBC Supervisory Committee Member Dr. Julian Guttman, Molecular Biology and Biochemistry, SFU Supervisory Committee Member iii Abstract The focus of this thesis is the characterization of the functions of the membrane contact sites (MCSs) between tubulobulbar complexes (TBCs) and the endoplasmic reticulum (ER). TBCs are double membrane endocytic invaginations that are endemic to Sertoli cells of the mammalian testis. They have been proposed to internalize intact intercellular junctions between late spermatids and Sertoli cells, allowing late spermatids to be released into the seminiferous tubule lumen. However, there is little …
has type: Thesis/Dissertation
date issued: 2021-05
is shown at: 10.14288/1.0396888
language: eng
degree discipline: Cell and Developmental Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/77889