Application of MagLev-based isolation technology for the rapid and sensitive concentration of influenza viruses
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Khalid, Muhammad Faizan
issued: 2021-02-24T17:59:36Z
issued: 2021
related degree: Master of Applied Science - MASc
degree grantor: University of British Columbia
description: Viruses are a fundamental branch of the tree of life. They are responsible for several biological processes and a major source of human and plant diseases. Despite their relevance to life however, most techniques to study them are dependent on indirect and invasive methods like PCR or immunological and cytological assays which require large numbers of whole viruses to function reliably. This is also true for the influenza virus which has caused several pandemics in human history. Many established techniques in virology additionally require extensive storage and intermediary steps to preserve and increase viral titters for analysis. These steps impose constraints on the process of detection and isolation while also destroying virus particles. To reduce the constraints imposed by things like primer bias, storage conditions and virus host interactions during enumeration steps, in this thesis I present a Magnetic Levitation (MagLev) based technique for the isolation and detection of influenza viruses. My main objective was to assess the performance of Magnetic Levitation (MagLev) based techniques for the sensitive and rapid isolation of intact influenza viruses in biological samples. The results show that influenza virions are levitated within a MagLev systems column consistently and distinctly using either Gadobutrol or Super Paramagnetic Iron Oxide Nanoparticle (SPION) solutions (in the MagLev) but do not produce a reliable visible marker for identification. However, the pure influenza A viral stock used in the experiment did produce a visualization in two trials which was used to determine a first ever direct MagLev based estimate of influenza A viral density (~ 0.978 ± 0.02 g/cm³). Which is notably lower than previous estimates of viral density (1.014-1.265 g/cm³) obtained through sucrose gradient based ultracentrifugation. Additionally, the results of viral quantification via Q-RTPCR and Tissue Culture Infectious Dose 50 (TCID50) assays corroborated the evidence of…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/77369
note: Application of MagLev-based isolation technology for the rapid and sensitive concentration of influenza viruses by Muhammad Faizan Khalid B.Sc., The University of British Columbia Okanagan, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF APPLIED SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Mechanical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) February 2021 © Muhammad Faizan Khalid, 2021 ii The undersigned certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis entitled: Application of a MagLev-based isolation technology for rapid and sensitive detection of influenza viruses Submitted by Muhammad Faizan Khalid in partial fulfilment of the requirements of The degree of: Master of Applied Science in Engineering Dr. Sepideh Pakpour, School of Engineering, Faculty of Applied Science: Mechanical Supervisor, Assistant Professor Dr. Isaac Li, IKBSAS: Chemistry Supervisory Committee Member, Assistant Professor Dr. Mohammad Hossein Zarifi, School of Engineering, Faculty of Applied Science: Electrical Supervisory Committee Member, Assistant Professor Dr. Andrew Jirasek, IKBSAS: Physics University Examiner, Professor iii Abstract Viruses are a fundamental branch of the tree of life. They are responsible for several biological processes and a major source of human and plant diseases. Despite their relevance to life however, most techniques to study them are dependent on indirect and invasive methods like PCR or immunological and cytological assays which require large numbers of whole viruses to function reliably. This is also true for the influenza virus which has caused several pandemics in human history. Many established techniques in virology additionally require extensive storage and intermediary steps to preserve and increase viral titters for analysis. These steps impose constraints on the process of detection and isolation while also destroying virus particles. To …
has type: Thesis/Dissertation
date issued: 2021-05
is shown at: 10.14288/1.0395955
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-ShareAlike 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-sa/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/77369