Designer probiotic supplementation outcomes on high fat diet
department or school: Science, Faculty of
data provider: DSpace
degree campus: UBCO
creator: Dadlani, Hansika
issued: 2021-08-31T07:00:00Z
issued: 2021
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: High fat diets (HFDs) cause insulin resistance, oxidative stress, inflammation, all of which are linked with gut microbial dysbiosis. As a therapy, probiotics can reduce inflammation, oxidative stress and insulin resistance under such conditions. However, for these protective effects, probiotic microbes need to colonize and persist in the gut, especially under conditions of oxidative insult and inflammation. Naturally occurring probiotics have fared poorly thus far. To overcome this challenge, two probiotics, BioColoniz & BioPersist, were created in Dr. Gibson’s laboratory from Lactobacillus reuteri and Escherichia coli Nissle 1917 (EcN) parent strains respectively. BioColoniz is bio-engineered to adhere and colonize the gut while BioPersist possesses a survival advantage by expressing a gene that confers resistance to ambient oxidative insult. We hypothesized that these designer probiotics could improve insulin insensitivity induced by HFDs. C57/Bl6 mice were fed a 40% energy HFD for 6 weeks. Mice were either not gavaged or gavaged with the one of the two probiotics during the study. Subsequently, analysis of BioPersist and Biocoloniz livers revealed lowered oxidative stress biomarker nitrotyrosine. BioPersist livers also demonstrated increased antioxidant (glutathione peroxidase-4) and reduced inflammatory biomarkers (inducible nitric oxide synthase and tumor necrosis factor-α). Mice administered BioPersist also gained the least amount of weight on HFD and demonstrated improved glucose tolerance tests. Improved phosphorylation and abundance of insulin/IGF-I receptor β in both BioPersist and BioColoniz livers were also noted. Furthermore, BioPersist livers had elevated phosphorylation of Akt, a key insulin signaling molecule. Overall, we show an improvement of in vivo glucose homeostasis associated with lower inflammation and oxidative stress in the liver with designer probiotics following a high fat diet. These results indicate that bio-engineered probiotics might…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/77270
note: DESIGNER PROBIOTIC SUPPLEMENTATION OUTCOMES ON HIGH FAT DIET by Hansika Dadlani B.Tech, Vellore Institute of Technology, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Biochemistry and Molecular Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) February 2021 © Hansika Dadlani, 2021 ii The following individuals certify that they have read, and recommend to the for acceptance, the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Designer probiotic supplementation outcomes on high fat diet submitted by Hansika Dadlani in partial fulfillment of the requirements for the degree of Master of Science Dr. Sanjoy Ghosh, Faculty of Science Supervisor Dr. Susan Murch, Faculty of Science Supervisory Committee Member Dr. Michael Deyholos, Faculty of Science Supervisory Committee Member Dr. Angelina Devlin, Faculty of Medicine University Examiner iii Abstract High fat diets (HFDs) cause insulin resistance, oxidative stress, inflammation, all of which are linked with gut microbial dysbiosis. As a therapy, probiotics can reduce inflammation, oxidative stress and insulin resistance under such conditions. However, for these protective effects, probiotic microbes need to colonize and persist in the gut, especially under conditions of oxidative insult and inflammation. Naturally occurring probiotics have fared poorly thus far. To overcome this challenge, two probiotics, BioColoniz & BioPersist, were created in Dr. Gibson’s laboratory from Lactobacillus reuteri and Escherichia coli Nissle 1917 (EcN) parent strains respectively. BioColoniz is bio-engineered to adhere and colonize the gut while BioPersist possesses a survival advantage by expressing a gene that confers resistance to ambient oxidative insult. We hypothesized that these designer probiotics could improve insulin insensitivity induced by HFDs. C57/Bl6 mice were fed a 40% energy HFD for 6 weeks. Mice were …
has type: Thesis/Dissertation
date issued: 2021-05
is shown at: 10.14288/1.0395848
language: eng
degree discipline: Biochemistry and Molecular Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/77270