Developing a Lob-On-a-Chip platform for manipulation and separation of microparticles
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Dalili Shoaei, Arash
issued: 2020-12-23T22:46:10Z
issued: 2020
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Cell/particle separation has a wide range of applications in environmental monitoring (e.g., air/water purification), food industry (e.g., microfiltration for improving food quality), oil and gas (e.g., particle separation in cyclones), and medicine/biotechnology (e.g., isolation of circulating tumor cells). Conventional procedures for separation purposes require costly infrastructure and several operational steps which are time-consuming and associated with human error. In the past two decades, there has been a tendency to develop microfluidic techniques, so-called lab-on-a-chip (LOC), to miniaturize and automate these procedures. Differences in the physical, electrical and biological properties of target cells/particles from the other components present in the fluid samples are crucial in the development of such microfluidic-based separation techniques. One of these techniques, which uses dielectrophoresis (DEP) force, is capable of the electric-based manipulation of noncharged particles in a non-uniform electric. Despite the general success, DEP still has not reached its full potential for high throughput operations. This thesis aims at studying the effect of several parameters on the performance of DEP-based microfluidic devices. In essence, prior to the separation process, particles need to be focused in a narrow region. A common approach is sheath-assisted focusing, which is numerically studied in this thesis to report the effective factors on the focusing width. COMSOL Multiphysics modeling is used to find the effect of the geometry of the electrodes (width and spacing) on the resulting DEP force. The effect of the applied voltage, channel height on the DEP-based manipulation of particles is investigated and it is shown that higher voltages and lower channel heights lead to more effective DEP manipulation. Furthermore, the effect of the length and width of the channel, the deflection angle of the electrodes, the volumetric throughput, and the particle size on…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/76870
note: DEVELOPING A LAB-ON-A-CHIP PLATFORM FOR MANIPULATION AND SEPARATION OF MICROPARTICLES by Arash Dalili Shoaei B.Sc., Amirkabir University of Technology, 2012 M.Sc., Amirkabir University of Technology, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Mechanical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) December 2020 © Arash Dalili, 2020 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Developing a Lob-On-a-Chip platform for manipulation and separation of microparticles submitted by Arash Dalili Shoaei in partial fulfillment of the requirements of the degree of Doctor of Philosophy . Dr. Mina Hoorfar, School of Engineering, The University of British Columbia Supervisor Dr. Homayoun Najjaran, School of Engineering, The University of British Columbia Supervisory Committee Member Dr. Keekyoung Kim, Department of Mechanical and Manufacturing Engineering, The University of Calgary Supervisory Committee Member Dr. Liwei Wang, School of Engineering, The University of British Columbia University Examiner Dr. Ali Dolatabadi, Department of Mechanical and Industrial Engineering, Concordia University External Examiner iii Abstract Cell/particle separation has a wide range of applications in environmental monitoring (e.g., air/water purification), food industry (e.g., microfiltration for improving food quality), oil and gas (e.g., particle separation in cyclones), and medicine/biotechnology (e.g., isolation of circulating tumor cells). Conventional procedures for separation purposes require costly infrastructure and several operational steps which are time-consuming and associated with human error. In the past two decades, there has been a tendency to develop microfluidic techniques, so-called lab-on-a-chip (LOC), to miniaturize and automate these procedures. Difference…
has type: Thesis/Dissertation
date issued: 2021-02
is shown at: 10.14288/1.0395367
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/76870