Design and fabrication of solvent compatible polymer microfluidic chips and its application to particle production and drug delivery
department or school: Pharmaceutical Sciences, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Geczy, Reka
issued: 2020-10-28T20:07:23Z
issued: 2020
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Despite the recent advancements in the field of microfluidics, the potential of rapid development is often limited due to the inherent challenges posed by the materials used for microfluidic device fabrication. For drug delivery applications, there is a need to identify an optimal material that is cost-effective, compatible with ‘soft-lithography,’ easily replica molded, and resistant to harsh solvents. The family of thiol-ene polymers hold promise as an inexpensive and easy-to-produce alternative. This material shows good chemical compatibility with most organic solvents but falls short for chlorinated solvents which are often used for pharmaceutical applications. Thus, the research presented in this thesis aimed to develop a solvent compatible thiol-ene platform for rapid and cost-effective fabrication of microfluidic chips with a focus on drug delivery applications. This work initially shows the rendering of thiol-ene polymers chloroform compatible in order to open new prototyping avenues for drug delivery purposes. The approach is simple and effective, resulting in a 50-fold increase in chloroform compatibility, allowing for the operation of microfluidic chips in chloroform for several days without any discernible deformation. Next, this thesis shows the novel preparation of small (1-2 μm), monodispersed polylactic acid (PLA) microspheres, utilizing chlorinated solvents for their synthesis. This work presents a simple microfluidic chip design achievable in all microfluidic fabrication labs and relies on flow manipulations to shear of droplets well under the often-regarded minimum size limits. The prepared particles show high monodispersity and significant loading with magnetite nanoparticles; hence, hold promise for magnetically targeted drug delivery. In addition to droplet production, thiol-enes are suited for the bulk precipitation of uniform nanoparticles. The final work presented here focuses on siRNA loading within the lipid-polymer hybrid nanoparticles. T…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/76387
note: DESIGN AND FABRICATION OF SOLVENT COMPATIBLE POLYMER MICROFLUIDIC CHIPS AND ITS APPLICATION TO PARTICLE PRODUCTION AND DRUG DELIVERY by Reka Geczy M.S., University of Copenhagen, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Pharmaceutical Sciences) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) October 2020 © Reka Geczy, 2020 The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Design and Fabrication of Solvent Compatible Polymer Microfluidic Chips and its Application to Particle Production and Drug Delivery submitted by Reka Geczy in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Pharmaceutical Sciences Examining Committee: Anan Yaghmur, Department of Pharmacy, University of Copenhagen University Examiner Rizhi Wang, Department of Materials Engineering, The University of British Columbia University Examiner Winnie E. Svendsen, Technical University of Denmark External Examiner Principal supervisor: Urs O. Häfeli Professor Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC, Canada Co-supervisors: Jörg P. Kutter Professor Department of Pharmacy University of Copenhagen, Copenhagen, Denmark Camilla Foged Professor Department of Pharmacy University of Copenhagen, Copenhagen, Denmark Assessment committee: Anan Yaghmur Associate Professor Department of Pharmacy University of Copenhagen, Copenhagen, Denmark Winnie E. Svendsen Professor Department of Biotechnology and Biomedicine Technical University of Denmark, Kgs. Lyngby, Denmark Rizhi Wang Professor Department of Materials Engineering The University of British Columbia, Vancouver, BC, Canada Preface This dissertation is formatted in accordance with the regulatio…
has type: Thesis/Dissertation
date issued: 2020-11
is shown at: 10.14288/1.0394834
language: eng
degree discipline: Pharmaceutical Sciences
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/76387