Radiation pneumonitis assessed in a mouse model of partial-body irradiation with minimal bone marrow sparing
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Computer Science, Mathematics, Physics and Statistics, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Fuentes, Alejandra Maria
issued: 2020-09-18T14:09:06Z
issued: 2020
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Purpose: Accidental exposure to high-dose ionizing radiation may lead to significant injuries to multiple organs. Herein, this thesis established a mouse model of high-dose partial-body irradiation with minimal bone marrow shielding to model the progression, severity, and clinical features of lung injury consequent of an accidental scenario and determine whether radiation-induced hematopoietic system depletion affects the pulmonary response. Methods: Female C3H/HeJ mice were irradiated in a single fraction, using a 6 MV Flattening-Filter-Free beam on a Varian TrueBeam LINAC. The following experimental groups were assessed: (1) 8 Gy partial-body irradiation (PBI), sparing the tibiae, ankles, and feet plus an 8 Gy simultaneous-infield-boost (SIB) to the lung to elicit lung damage, (2) 16 Gy Whole-thorax lung irradiation (WTLI), (3) 8 Gy PBI, and (4) untreated controls. Mice were monitored weekly and euthanized upon the presentation of respiratory distress or at the 16-week experimental endpoint. At sacrifice, blood and lung tissue were collected for Complete Blood Count and histopathology. Additionally, subsets of mice were sacrificed one-week post-treatment to assess acute hematopoietic cell depletion. Results: Treatment with 8 Gy partial-body irradiation depleted hematopoietic cell counts, specifically leukocytes and platelets, measured at one week post-irradiation, significantly more than 16 Gy WTLI. The survival time of mice treated with 16 Gy WTLI was not significantly different from that of mice given 8 Gy PBI plus 8 Gy lung SIB (p=0.17). Lung histology revealed that mice of both 16 Gy WTLI and 8 Gy PBI plus 8 Gy lung SIB groups developed a diffuse pneumonitis response; the severity score was not significantly different between these treatment groups (p=0.96). Finally, partial-body irradiation significantly reduced blood monocyte counts of mice at respiratory distress compared to levels in local whole-thorax irradiated mice but did not influence neutrophil count…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/76078
note: Radiation pneumonitis assessed in amouse model of partial-bodyirradiation with minimal bone marrowsparingbyAlejandra Maria FuentesB.Sc., Acadia University, 2018A THESIS SUBMITTED IN PARTIAL FULFILLMENT OFTHE REQUIREMENTS FOR THE DEGREE OFMASTER OF SCIENCEinThe College of Graduate Studies(Medical Physics)THE UNIVERSITY OF BRITISH COLUMBIA(Okanagan)September 2020c© Alejandra Maria Fuentes, 2020The following individuals certify that they have read, and recommendto the College of Graduate Studies for acceptance, a thesis/dissertation en-titled:Radiation pneumonitis assessed in a mouse model of partial-body irradiationwith minimal bone marrow sparingsubmitted by Alejandra Maria Fuentes in partial fulfilment of the re-quirements of the degree of Master of ScienceDr. Christina Haston, Irving K. Barber Faculty of ScienceSupervisorDr. Michelle Hilts, Irving K. Barber Faculty of ScienceSupervisory Committee MemberDr. Derek Hyde, Irving K. Barber Faculty of ScienceSupervisory Committee MemberDr. Neil Eves, Faculty of Health and Social DevelopmentUniversity ExamineriiAbstractPurpose: Accidental exposure to high-dose ionizing radiation may leadto significant injuries to multiple organs. Herein, this thesis established amouse model of high-dose partial-body irradiation with minimal bone mar-row shielding to model the progression, severity, and clinical features of lunginjury consequent of an accidental scenario and determine whether radiation-induced hematopoietic system depletion affects the pulmonary response.Methods: Female C3H/HeJ mice were irradiated in a single fraction, us-ing a 6 MV Flattening-Filter-Free beam on a Varian TrueBeam LINAC. Thefollowing experimental groups were assessed: (1) 8 Gy partial-body irradia-tion (PBI), sparing the tibiae, ankles, and feet plus an 8 Gy simultaneous-infield-boost (SIB) to the lung to elicit lung damage, (2) 16 Gy Whole-thoraxlung irradiation (WTLI), (3) 8 Gy PBI, and (4) untreated controls. Micewere monitored weekly and euthanized up…
has type: Thesis/Dissertation
date issued: 2020-11
is shown at: 10.14288/1.0394422
language: eng
degree discipline: Medical Physics
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/76078