Development of 3D functional brain tissue model
department or school: Applied Science, Faculty of
department or school: Mechanical Engineering, Department of
data provider: DSpace
degree campus: UBCO
creator: Samanipour, Roya
issued: 2019-12-23T18:32:30Z
issued: 2019
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Nervous system disorders including acute traumatic injuries, neurodegenerative diseases, and neurodevelopmental disorders are estimated to affect more than one billion people worldwide. Study and understanding the development of the human nervous system and exposing the mechanisms of mental disorders has greatly been limited due to the restricted access to the functional human brain tissue. 3D in vitro organ models has recently shown to be a powerful tool for biological and medical studies. These models, however, require special 3D construction of cell and extracellular matrixes that are often hard to achieve with conventional fabrication approaches. Bioprinting technique has emerged as potent platform to fabricate these complex 3D models. Here, state-of-art stem cell-based 3D in vitro brain models that recapitulate the geometrical complexity of the brain are developed using 3D bioprinting. The model is developed based on two cell types, neural stem cell and primary astrocytes. To create the model, a high-throughput biofabrication strategy based on embedded 3D bioprinting technology is designed, developed and characterized. Protocols, culture media, bioinks and biomaterials used are tuned and optimized to increase cell viability, enhance cell activity and promote neural stem cell differentiation. The procedures are optimized through a series of 2D and 3D studies and finally, the 3D bioprinted brain in vitro model is carried out. Keywords: Brain tissue, Neural stem cells, Bioprinting, Differentiation
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/72935
note: i Development of 3D functional brain tissue model by Roya Samanipour B.Sc., The Shahid Chamran University, 2007 M.Sc., The Tarbiat Modares University, 2012 M.Sc., The University of British Columbia, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Mechanical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) December 2019 © Roya Samanipour, 2019 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Development of 3D functional brain tissue model submitted by Roya Samanipour in partial fulfillment of the requirements of the degree of DOCTOR OF PHILOSOPHY . Dr. Mina Hoorfar (Supervisor) Dr. SuRyon Shin (Co-supervisor) Supervisor Dr. Homayoun Najjaran Supervisory Committee Member Dr. Keekyoung Kim Supervisory Committee Member Dr. Richard Klukas University Examiner Dr. Khashayar khoshmanesh External Examiner iii Abstract Nervous system disorders including acute traumatic injuries, neurodegenerative diseases, and neurodevelopmental disorders are estimated to affect more than one billion people worldwide. Study and understanding the development of the human nervous system and exposing the mechanisms of mental disorders has greatly been limited due to the restricted access to the functional human brain tissue. 3D in vitro organ models has recently shown to be a powerful tool for biological and medical studies. These models, however, require special 3D construction of cell and extracellular matrixes that are often hard to achieve with conventional fabrication approaches. Bioprinting technique has emerged as potent platforms to fabricate these complex 3D models. Here, state-of-art stem cell-based 3D in vitro brain models that recapitulate the geometrical complexity of the brain are developed using 3D bioprinting. The model is developed based on two cell types, neural stem cell…
has type: Thesis/Dissertation
date issued: 2019-02
is shown at: 10.14288/1.0387307
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/72935