Prevalence and characterization of Campylobacter isolated from chicken products in metro Vancouver
department or school: Land and Food Systems, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Tzanidis, Anestis Marios
issued: 2021-01-31T08:00:00Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Campylobacter has been recognized as the leading cause of human gastrointestinal diseases with C. jejuni and C. coli being responsible for ~90% and ~10% of human campylobacteriosis, respectively. In this study, we determined the prevalence and characteristics of these two thermotolerant Campylobacter species in retail chicken in metro Vancouver, Canada. Over 200 chicken samples were collected from different districts and tested for Campylobacter contamination using modified Charcoal Cefoperazone Deoxycholate Agar after enrichment in Preston broth. A multiplex polymerase chain reaction (m-PCR) assay was applied to identify C. jejuni and C. coli strains, followed by antimicrobial susceptibility tests using the broth microdilution method. Aminoglycosides, lincosamides, macrolides, quinolones, tetracyclines and phenicols were the major antimicrobial classes used to determine the antimicrobial resistance patterns of the isolates. Finally, we performed multilocus sequence typing to investigate the population structure and their relationship with Canadian human isolates. A total of 34% of the samples were tested positive for C. jejuni (80%) and C. coli (20%) and the majority were resistant to nalidixic acid at 60% and 69%, followed by resistance to ciprofloxacin at 51.1% and 38.4% for C. jejuni and C. coli, respectively. We did not discover any multi-drug resistant Campylobacter belonging to either species. Nevertheless, 17.8% of C. jejuni and 38.5% of C. coli were resistant to ciprofloxacin, nalidixic acid, tetracycline and doxycycline at the same time. The chicken isolates showed significant genetic diversity with 9 C. jejuni sequence types (STs) belonging to six commonly identified clonal complexes (ST-21, ST-42, ST-48, ST-283, ST-343 and ST-353) and 9 C. coli isolates belonging to one clonal complex (ST-828). This study shed light on the levels, population structure and antimicrobial resistance of C. jejuni and C. coli isolated from chicken products in Vancouver, empha…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/72855
note: PREVALENCE AND CHARACTERIZATION OF CAMPYLOBACTER ISOLATED FROM CHICKEN PRODUCTS IN METRO VANCOUVER by Anestis Marios Tzanidis B.Sc., Aristotle University of Thessaloniki, 2006 M.Sc., University of Thessaly, 2008 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (FOOD SCIENCE) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) December 2019 © Anestis Marios Tzanidis, 2019 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis entitled: Prevalence and characterization of Campylobacter isolated from chicken products in metro Vancouver submitted by Anestis Marios Tzanidis in partial fulfillment of the requirements for the degree of Master of Science in Food Science Examining Committee: Xiaonan Lu Supervisor Savvas Hatzikiriakos Supervisory Committee Member Supervisory Committee Member Vivien Measday Additional Examiner iii Abstract Campylobacter has been recognized as the leading cause of human gastrointestinal diseases with C. jejuni and C. coli being responsible for ~90% and ~10% of human campylobacteriosis, respectively. In this study, we determined the prevalence and characteristics of these two thermotolerant Campylobacter species in retail chicken in metro Vancouver, Canada. Over 200 chicken samples were collected from different districts and tested for Campylobacter contamination using modified Charcoal Cefoperazone Deoxycholate Agar after enrichment in Preston broth. A multiplex polymerase chain reaction (m-PCR) assay was applied to identify C. jejuni and C. coli strains, followed by antimicrobial susceptibility tests using the broth microdilution method. Aminoglycosides, lincosamides, macrolides, quinolones, tetracyclines and phenicols were the major antimicrobial classes used to determine the antimicrobial resistance patterns of the isolates. Finally, we performed multilocu…
has type: Thesis/Dissertation
date issued: 2020-05
is shown at: 10.14288/1.0387217
language: eng
degree discipline: Food Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/72855