The role of glutathione peroxidase 1 in the heart following high fat feeding
department or school: Arts and Sciences, Irving K. Barber School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Renaud, Lisa
issued: 2019-10-21T15:32:48Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Over the last century, the composition of dietary fats has changed in Western countries leading to an increased ratio of n-6:n-3 polyunsaturated fatty acid (PUFA) consumption. It has been suspected that this altered ratio may contribute to the increase in incidence of cardiovascular diseases in Western countries. One of the classical ways how diet can impact diseases is through increasing oxidative stress. Previous studies have shown that linoleic acid (LA), a common dietary n-6 PUFA, is able to induce oxidative stress. Oxidative stress in the heart can be mitigated by antioxidants. In this regard, glutathione peroxidase 1 (GPx1) is an important antioxidant involved in protecting the heart. My project was to investigate the role of GPx1, in protecting the heart especially following a high n-6 fatty acid Western diet. The first objective was to investigate the effects of high fat diets in vivo in GPx1 deficient mouse hearts (Chapter 1). At the onset, we determined that GPx1⁺/- mouse hearts had lower mitochondrial copy number indicating metabolic abnormality. Upon feeding of various diets, we established that a GPx1 heterozygous KO does not affect linoleic acid induced cardiotoxicity in mice Further, GPx1⁺/- mouse hearts did not have reduced cardiac function, thereby indicating that even in reduced amounts, GPx1 is able to provide protection. Regarding antioxidants the GPx1+/- mouse hearts had upregulated gene expression of other antioxidants such as GPx4, superoxide dismutase (SOD) 1, SOD2, and catalase when compared to the wildtype control mice, indicating that alternate antioxidants did maintain antioxidant status in these mice. The second objective was to investigate the interactions between GPx1 and fatty acids in vitro on antioxidant regulation in H9c2 cardiomyocytes, one of the main heart cell types, in causing cell death (Chapter 2). GPx1 overexpression increased cellular uptake of neutral red indicating higher cell number. However, with incubation with fatty …
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/71987
note: THE ROLE OF GLUTATHIONE PEROXIDASE 1 IN THE HEART FOLLOWING HIGH FAT FEEDING by Lisa Renaud B.Sc., University of British Columbia Okanagan, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The College of Graduate Studies (Biochemistry and Molecular Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) October 2019 © Lisa Renaud, 2019 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: INVESTIGATING THE ROLE OF GLUTATHIONE PEROXIDASE 1 IN THE HEART FOLLOWING HIGH FAT FEEDING submitted by Lisa Renaud in partial fulfillment of the requirements of the degree of Master of Science. Dr. Sanjoy Ghosh, Irving K. Barber School of Arts and Sciences, University of British Columbia Okanagan Supervisor Dr. Mark Rheault, Irving K. Barber School of Arts and Sciences, University of British Columbia Okanagan Supervisory Committee Member Dr. Michael Deyholos, Irving K. Barber School of Arts and Sciences, University of British Columbia Okanagan Supervisory Committee Member Dr. Ismail Laher, Anaestheology, Pharmacology and Therapeautics, University of British Columbia Vancouver University Examiner External Examiner Additional Committee Members include: Supervisory Committee Member Supervisory Committee Member iii Abstract Over the last century, the composition of dietary fats has changed in Western countries leading to an increased ratio of n-6:n-3 polyunsaturated fatty acid (PUFA) consumption. It has been suspected that this altered ratio may contribute to the increase in incidence of cardiovascular diseases in Western countries. One of the classical ways how diet can impact diseases is through increasing oxidative stress. Previous studies have shown that linoleic acid (LA), a common dietary n-6 PUFA, is able to induce oxidative stress. Oxidative stress in the heart can be mitigated by antioxidants. In this regard, glutathione …
has type: Thesis/Dissertation
date issued: 2019-11
is shown at: 10.14288/1.0384573
language: eng
degree discipline: Biochemistry and Molecular Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/71987